Cicculli Vincent, Oscar Maestrini, Casabianca Francois, Villechenaud Natacha, Charrel Remi, de Lamballerie Xavier, Falchi Alessandra
Laboratoire de Virologie, Université de Corse-Inserm, 20250 EA7310 Corte, France.
Laboratoire de Recherches sur le Développement de l'Elevage (Research Laboratory for the Development of Livestock), 20250 UR045 Corte, France.
Pathogens. 2019 Sep 4;8(3):138. doi: 10.3390/pathogens8030138.
To obtain a better understanding of the current magnitude of tick-borne rickettsioses in Corsica, we used molecular methods to characterize the occurrence of spp. in ixodid ticks collected from domestic and wild animals. The presence of spp. was evaluated using real-time polymerase chain reaction targeting the gene and by sequencing of and partial genes for species identification and phylogenetic analysis. Infection rates were calculated as the maximum-likelihood estimation (MLE) with 95% confidence intervals (CI). In total, 1117 ticks belonging to four genera (, , , and ) were collected from cattle, sheep, wild boars, and companion animals during July-August 2017 and July 2018-January 2019. Overall, DNA was detected in 208 of 349 pools of ticks (MLE = 25.6%, 95% CI: 22.6-28.8%). The molecular analysis revealed five different rickettsial species of the spotted-fever group (SFG). We highlighted the exclusive detection of Ri. barbariae in and of in was detected in collected from wild boars. This study provides the first evidence of the presence of in ticks isolated from a dog in Corsica. In conclusion, our data revealed wide dispersal of SFG Rickettsiae and their arthropod hosts in Corsica, highlighting the need for surveillance of the risk of infection for people living and/or working close to infected or infested animals.
为了更好地了解科西嘉岛蜱传立克次体病的当前流行程度，我们采用分子方法对从家畜和野生动物采集的硬蜱中 spp. 的出现情况进行了特征分析。使用针对 基因的实时聚合酶链反应以及对 和 部分基因进行测序来评估 spp. 的存在情况，以进行物种鉴定和系统发育分析。感染率通过最大似然估计（MLE）及 95% 置信区间（CI）进行计算。2017 年 7 - 8 月以及 2018 年 7 月至 2019 年 1 月期间，共从牛、羊、野猪和伴侣动物身上采集了 1117 只属于四个属（, ,, 和 ）的蜱。总体而言，在 349 组蜱中有 208 组检测到了 DNA（MLE = 25.6%，95% CI：22.6 - 28.8%）。分子分析揭示了斑点热群（SFG）的五种不同立克次体物种。我们强调在 中独家检测到了 Ri. barbariae，在 中检测到了 ，在从野猪采集的 中检测到了 。本研究提供了科西嘉岛一只狗身上分离出的 蜱中存在 的首个证据。总之，我们的数据揭示了 SFG 立克次体及其节肢动物宿主在科西嘉岛广泛分布，突出了对生活和/或工作在受感染或有蜱虫侵扰动物附近人群感染风险进行监测的必要性。
