Damgaard Christian, Danielsen Anne Katrine, Enevold Christian, Massarenti Laura, Nielsen Claus Henrik, Holmstrup Palle, Belstrøm Daniel
Section for Periodontology and Microbiology, Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Institute for Inflammation Research, Center for Rheumatology and Spine Diseases, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.
J Oral Microbiol. 2019 Aug 9;11(1):1653123. doi: 10.1080/20002297.2019.1653123. eCollection 2019.
: To characterize the salivary microbiota of patients with aggressive periodontitis, patients with chronica periodontitis and orally healthy individuals. : A total of 81 unstimulated saliva samples from aggressive periodontitis patients (n = 31), chronic periodontitis patients (n = 25), and orally healthy controls (n = 25) were examined. The V1-V3 region of the gene was sequenced with Illumina® MiSeq, and sequences were annotated to the expanded Human Oral Microbiome Database (eHOMD). : A mean percentage of 97.6 (range: 89.8-99.7) of sequences could be identified at species level. Seven bacterial species, including were identified with significantly higher relative abundance in saliva from aggressive periodontitis patients than in saliva from orally healthy controls. Salivary abundance of could discriminate aggressive (AUC: 0.80, p = 0.0001) and chronic periodontitis (AUC: 0.72, p = 0.006) from healthy controls. Likewise, salivary presence of was significantly associated with aggressive (p < 0.0001, RR: 8.1 (95% CI 2.1-31.2)) and chronic periodontitis (p = 0.002, RR: 6.5 (95% CI: 1.6-25.9)). : Salivary presence and relative abundance of associate with aggressive and chronic periodontitis, but do not discriminate between aggressive and chronic periodontitis.
为了描述侵袭性牙周炎患者、慢性牙周炎患者和口腔健康个体的唾液微生物群。
共检测了81份来自侵袭性牙周炎患者（n = 31）、慢性牙周炎患者（n = 25）和口腔健康对照者（n = 25）的非刺激性唾液样本。使用Illumina® MiSeq对16S rRNA基因的V1-V3区域进行测序，并将序列注释到扩展的人类口腔微生物组数据库（eHOMD）。
平均有97.6%（范围：89.8 - 99.7）的序列可在物种水平上鉴定出来。包括某种细菌在内的7种细菌在侵袭性牙周炎患者唾液中的相对丰度显著高于口腔健康对照者唾液中的相对丰度。该细菌的唾液丰度可区分侵袭性牙周炎（AUC：0.80，p = 0.0001）和慢性牙周炎（AUC：0.72，p = 0.006）与健康对照者。同样，该细菌在唾液中的存在与侵袭性牙周炎（p < 0.0001，RR：8.1（95% CI 2.1 - 31.2））和慢性牙周炎（p = 0.002，RR：6.5（95% CI：1.6 - 25.9））显著相关。
该细菌在唾液中的存在和相对丰度与侵袭性和慢性牙周炎相关，但不能区分侵袭性和慢性牙周炎之间的差异。
