Bjorner Marianne, Zhu Lida
Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA, USA.
Pathobiol Aging Age Relat Dis. 2019 Jul 26;9(1):1647400. doi: 10.1080/20010001.2019.1647400. eCollection 2019.
Hematologic analysis is an efficient and valuable tool for real-time health monitoring and immune analysis in mouse aging studies. However, many frequently used blood sampling techniques in mice are incompatible with continuous monitoring with increasing age, as they may involve anesthesia, cause severe stress, or require a high volume of blood. This technical report describes a convenient relatively noninvasive procedure for counting white blood cells in C57Bl/6 mice by an optimized tail blood collection method followed by Wright-Giemsa and Türk staining. This technique can be performed on unanesthetized mice in less than 1 min with minimal stress using only a few microliters of blood. White blood cell analysis can include but is not limited to total and differential white blood cell count and cell morphology. The low blood volume collected is ideal for aging mice in longer-term experiments requiring regular and continuous monitoring.
血液学分析是小鼠衰老研究中实时健康监测和免疫分析的一种有效且有价值的工具。然而，小鼠中许多常用的采血技术与随着年龄增长的连续监测不兼容，因为它们可能涉及麻醉、造成严重应激或需要大量血液。本技术报告描述了一种方便且相对无创的程序，通过优化的尾静脉采血方法，随后进行瑞氏-吉姆萨和 Türk 染色，对 C57Bl/6 小鼠的白细胞进行计数。该技术仅需使用几微升血液，在不到 1 分钟的时间内即可在未麻醉的小鼠身上进行，且应激极小。白细胞分析可包括但不限于白细胞总数和分类计数以及细胞形态。采集的少量血液对于需要定期和连续监测的长期实验中的衰老小鼠来说非常理想。
