Dürschlag M, Würbel H, Stauffacher M, Von Holst D
Department of Animal Physiology, University of Bayreuth, Germany.
Physiol Behav. 1996 Dec;60(6):1565-8. doi: 10.1016/s0031-9384(96)00307-1.
Twenty years ago, tail incision for collecting serial blood samples from mice was introduced as a new technique. Despite a number of advantages over established methods, it has not become a frequently used technique. This report describes modifications of blood collection from mice by tail incision that allow obtaining rapid (1-1.5 min) serial blood samples (40-150 microliters) from unanaesthetized laboratory mice. Evaluation of corticosterone concentrations in the blood plasma from repeated samples indicated that subsequent samples were unaffected by the procedure. Furthermore, histopathological examination demonstrated that repeated bleeding did not cause any lasting harm to the animals. Blood collection by tail incision may, therefore, be of particular interest for studies that attempt to relate physiological measures to behavioral responses in laboratory mice, and may contribute to the refinement of animal experimentation according to the principles of the Three Rs.
二十年前，从小鼠尾部切口采集系列血样作为一项新技术被引入。尽管与现有方法相比有诸多优点，但它尚未成为一种常用技术。本报告描述了通过尾部切口从小鼠采集血液的改进方法，该方法能从未麻醉的实验小鼠中快速（1 - 1.5分钟）采集系列血样（40 - 150微升）。对重复采集样本的血浆中皮质酮浓度的评估表明，后续样本不受该操作影响。此外，组织病理学检查表明，重复采血不会对动物造成任何持久伤害。因此，对于试图将生理指标与实验小鼠行为反应相关联的研究而言，尾部切口采血可能特别有意义，并且可能有助于根据“3R”原则优化动物实验。
