College of Food Science & Institute of Food Biotechnology, South China Agricultural University, Guangzhou, 510642, China.
Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou, 510642, China.
Appl Microbiol Biotechnol. 2019 Oct;103(19):7943-7952. doi: 10.1007/s00253-019-10101-z. Epub 2019 Sep 5.
In the present work, a novel cordycepic pigment was successfully isolated and identified from Cordyceps militaris, as well as named as cordycepene (CHNO), according to the long unsaturated conjugated polyene structural characteristic. Cordycepene is sensitive to light, high temperature (≥ 60 °C), and acidic condition (pH ≤ 3), but possesses high stability against metal ions, and under alkaline and neutral conditions. Cordycepene shows a comparable DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity at higher concentration (≥ 2 mg/mL) to vitamin C. Cordycepene promotes the growth of HSF (human skin fibroblast cell) after incubation for 72 h, and has an ability to repair the UV light-treated HSF cells. In addition, cordycepene increases the antioxidant activity (SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; CAT, catalase) and decreases MDA (malondialdehyde) level, indicating that cordycepene inhibits the photochemical senescence of HSF by enhancing the antioxidant defense system. The discovery of cordycepene can provide a basis for research on light incubation and the accumulation of yellow pigment (carotenoids) from C. militaris.
在本工作中，从蛹虫草中成功分离并鉴定出一种新型虫草素类色素，根据其长的不饱和共轭多烯结构特征，将其命名为虫草素（CHNO）。虫草素对光、高温（≥60°C）和酸性条件（pH≤3）敏感，但对金属离子稳定，且在碱性和中性条件下稳定。虫草素在较高浓度（≥2mg/mL）时，对 DPPH（1,1-二苯基-2-苦基肼基）自由基清除活性与维生素 C 相当。虫草素孵育 72 小时后促进 HSF（人皮肤成纤维细胞）的生长，并且具有修复 UV 光处理的 HSF 细胞的能力。此外，虫草素增加抗氧化活性（SOD，超氧化物歧化酶；GSH-Px，谷胱甘肽过氧化物酶；CAT，过氧化氢酶）并降低 MDA（丙二醛）水平，表明虫草素通过增强抗氧化防御系统抑制 HSF 的光化学衰老。虫草素的发现可为蛹虫草光照培养和黄色素（类胡萝卜素）积累的研究提供依据。
