He Rong-An, Huang Chen, Zheng Chun-Hui, Wang Jing, Yuan Si-Wen, Chen Bai-Xiong, Feng Kun
School of Bioengineering, Zunyi Medical University, Zhuhai, China.
State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
Front Microbiol. 2024 Oct 22;15:1437963. doi: 10.3389/fmicb.2024.1437963. eCollection 2024.
is a filamentous fungus used for both medicinal and culinary purposes. It exhibits a wide range of pharmacological activities due to its valuable contents of cordycepin, polysaccharides, carotenoids, terpenoids and other metabolites. However, strains are highly susceptible to irreversible degradation in agricultural production, which is often manifested as a prolonged color change period and a significant decrease in the production of secondary metabolites. UDP-glycosyltransferases are an important enzyme family that participates in the synthesis of terpenoids by performing the glycosylation of key residues of enzymes or molecules. However, few studies have focused on its effect on the regulation of metabolite production in . Therefore, in this study, we performed transcriptome analysis across four different developmental stages of to target the putative glycosyltransferase gene which plays important roles in metabolite production. We further constructed and screened a -overexpressing strain by -mediated infestation of spores. The major metabolite production of the wild-type and -overexpressing strains was determined after short-term shake-flask cultivation of mycelia. The results showed that the yields of carotenoids and polysaccharides in the mycelia of the -overexpressing strains were 3.8 and 3.4 times greater than those in the mycelia of the wild type, respectively ( < 0.01). The levels of intracellular and extracellular cordycepin produced by the overexpression strain were 4.4 and 8.0 times greater than those produced by the wild-type strain ( < 0.01). This suggests that the overexpression of in enhances the synthesis activities of the main enzymes related to metabolite production, which provides a guide for obtaining excellent recombinant strains of .
是一种用于医药和烹饪目的的丝状真菌。由于其含有宝贵的虫草素、多糖、类胡萝卜素、萜类化合物和其他代谢产物，它具有广泛的药理活性。然而，该菌株在农业生产中极易受到不可逆降解的影响，这通常表现为变色期延长和次生代谢产物产量显著下降。UDP-糖基转移酶是一个重要的酶家族，通过对酶或分子的关键残基进行糖基化参与萜类化合物的合成。然而，很少有研究关注其对该菌株代谢产物生产调控的影响。因此，在本研究中，我们对该菌株的四个不同发育阶段进行了转录组分析，以靶向推定的糖基转移酶基因，该基因在代谢产物生产中起重要作用。我们进一步通过农杆菌介导的孢子侵染构建并筛选了一个该基因过表达菌株。在对菌丝体进行短期摇瓶培养后，测定了野生型和该基因过表达菌株的主要代谢产物产量。结果表明，该基因过表达菌株菌丝体中类胡萝卜素和多糖的产量分别比野生型菌丝体高3.8倍和3.4倍（P < 0.01）。过表达菌株产生的细胞内和细胞外虫草素水平分别是野生型菌株的4.4倍和8.0倍（P < 0.01）。这表明该基因在该菌株中的过表达增强了与代谢产物生产相关的主要酶的合成活性，这为获得该菌株的优良重组菌株提供了指导。
