Beaulieu D, Ouellette M, Bergeron M G, Roy P H
Département de Biochimie, Faculté des Sciences et génie, Université Laval, Quebec, Canada.
Plasmid. 1988 Sep;20(2):158-62. doi: 10.1016/0147-619x(88)90020-0.
We isolated a 12.2-kb plasmid from two clinical strains of Branhamella catarrhalis and evaluated its distribution among other B. catarrhalis strains by colony hybridization experiments using the whole plasmid as a probe. Homology was detected with the two plasmid-bearing strains and also with a third B. catarrhalis strain named E7, which is plasmidless. Southern transfer analysis of total digested E7 DNA using the purified plasmid as a probe revealed a single band of hybridization, different from those observed in plasmid-bearing strains, for each restriction enzyme used. The region of the plasmid hybridizing with DNA of strain E7 was located within a 4.5-kb SstI-EcoRV fragment. No homology was noted between our B. catarrhalis plasmid and about 20 other strains of different genera tested by colony hybridization.
