Department of Pharmacy, Xiamen Medical College, Xiamen, China.
College of Biological Engineering, Jimei University, Xiamen, China.
J Food Biochem. 2019 Sep;43(9):e12981. doi: 10.1111/jfbc.12981. Epub 2019 Jul 21.
The chemical constituents in Trifolium repens L. were comprehensively studied by UPLC in this work, and a total number of 308 compounds were detected with 169 ones identified. The possible fragmentation pathways were proposed and fragmentation rules were summarized. On the basis of the concluded strategies, the characterized compounds could be classified into organic acids and their derivatives, alkaloids, amino acids, peptides, flavonoids, oligosaccharides, coumarins, and other types of compounds. This approach provided a rapid way for the identification of constituents in T. repens L., and even in other complex analytes. Among the separation and identification of the constituents, three compounds of great amount were isolated and characterized by NMR. The expression of iNOS and COX-2 in LPS-induced RAW 264.7 cells was suppressed by the pretreatment with three isolated constituents. The results implied they may potentially serve as a remedy for the therapy of inflammation. PRACTICAL APPLICATIONS: This work provided a rapid method for the identification of the complex analyte, which could be used in TCM, natural food and so on. The summarized fragmentation rule could be applied for the analysis of several types of compounds, such as organic acids and their derivatives, alkaloids, amino acids and peptides, flavonoids, oligosaccharides, coumarins, and so on. Most of natural plants contain these kinds of compounds, so these rules could have wide applications. Except the phytochemical investigation, T. repens L. displayed anti-inflammation activity according to the reported literature, and the three isolated constituents may potentially serve as a remedy for the therapy of inflammation referring to the result of this research.
本工作通过 UPLC 对三叶草中的化学成分进行了全面研究，共检测到 308 种化合物，其中鉴定了 169 种。提出了可能的断裂途径，并总结了断裂规律。在此总结策略的基础上，将所鉴定的化合物分为有机酸及其衍生物、生物碱、氨基酸、肽、黄酮类、低聚糖、香豆素和其他类型的化合物。这种方法为鉴定三叶草中的成分提供了一种快速的方法，甚至可以用于其他复杂的分析物。在成分的分离和鉴定中，通过 NMR 分离和鉴定了 3 种大量化合物。三种分离得到的化合物预处理可抑制 LPS 诱导的 RAW264.7 细胞中 iNOS 和 COX-2 的表达。结果表明，它们可能具有治疗炎症的潜力。实际应用：本工作为复杂分析物的鉴定提供了一种快速的方法，可用于中药、天然食品等领域。总结的断裂规律可用于分析有机酸及其衍生物、生物碱、氨基酸和肽、黄酮类、低聚糖、香豆素等多种类型的化合物。大多数天然植物都含有这些类型的化合物，因此这些规律具有广泛的应用。除了植物化学研究外，根据文献报道，三叶草具有抗炎活性，根据本研究的结果，三种分离得到的化合物可能具有治疗炎症的潜力。
