Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Food Hygiene and Environmental Health, Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto, Japan.
Microbiol Immunol. 2019 Dec;63(12):513-516. doi: 10.1111/1348-0421.12742. Epub 2019 Sep 23.
Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections in mammals, including humans, and are generally cultivated on Bordet-Gengou (BG) agar plates in laboratories. The medium requires animal blood as a supplement for better bacterial growth. However, using blood is problematic, as its constant supply is occasionally difficult because of the limited shelf-life. This study proposes modified BG agar plates supplemented with bovine serum albumin and fetal bovine serum as a simple and convenient medium that confers sufficient growth of bordetellae.
百日咳博德特氏菌、副百日咳博德特氏菌和支气管败血波氏杆菌会导致包括人类在内的哺乳动物发生呼吸道感染，通常在实验室的 Bordet-Gengou（BG）琼脂平板上进行培养。该培养基需要动物血液作为补充物，以促进更好的细菌生长。然而，使用血液存在问题，因为由于保质期有限，血液的持续供应偶尔会很困难。本研究提出了一种改良的 BG 琼脂平板，其中添加了牛血清白蛋白和胎牛血清，作为一种简单方便的培养基，可以为博德特氏菌提供足够的生长条件。
