Ruijs G J, Groenendijk T W, Biever M
Bacteriological Laboratory, Leyenburg Hospital, The Hague, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1991 Nov;10(11):974-8. doi: 10.1007/BF02005458.
The shelf life of prepared agar plates used for the isolation of Bordetella pertussis was studied. They contained Bordet-Gengou agar, Bordet-Gengou agar with cephalexin, Regan-Lowe agar, Regan-Lowe agar with cephalexin, or Regan-Lowe agar with both cephalexin and amphotericin B. Plates stored were compared to freshly prepared control plates for up to a maximum of 18 weeks. They were inoculated with clinical isolates of Bordetella pertussis, either in pure culture, or mixed with a defined oropharyngeal flora. Bordet-Gengou agar plates may be used, with proper storage at 4 degrees C in airtight-sealed plastic bags, for up to 10 weeks, Regan-Lowe agar plates for up to 14 weeks. Field studies are needed to substantiate our findings.
对用于分离百日咳博德特氏菌的制备好的琼脂平板的保质期进行了研究。这些平板含有博-金氏琼脂、含头孢氨苄的博-金氏琼脂、雷根-洛氏琼脂、含头孢氨苄的雷根-洛氏琼脂或含头孢氨苄和两性霉素B的雷根-洛氏琼脂。将储存的平板与新鲜制备的对照平板进行比较，最长达18周。用百日咳博德特氏菌的临床分离株进行接种，接种方式为纯培养，或与确定的口咽部菌群混合。博-金氏琼脂平板在4℃下妥善保存在密封塑料袋中，可使用长达10周，雷根-洛氏琼脂平板可使用长达14周。需要进行现场研究来证实我们的发现。
