Kurzynski T A, Boehm D M, Rott-Petri J A, Schell R F, Allison P E
Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison 53706.
J Clin Microbiol. 1988 Dec;26(12):2661-3. doi: 10.1128/jcm.26.12.2661-2663.1988.
Culture and fluorescent-antibody methods for detection of Bordetella species were evaluated by two state public health laboratories. Field-inoculated plates of Regan-Lowe agar medium were most useful if incubation was initiated on the day of collection. Regan-Lowe and Bordet-Gengou media were comparable for subculturing nasopharyngeal specimens that were transported and enriched in half-strength Regan-Lowe agar. Maximum sensitivity was achieved when the media were used in parallel. Fluorescent-antibody-stained smears of nasopharyngeal specimens were more sensitive for detection of Bordetella pertussis than for detection of Bordetella parapertussis. The fluorescent-antibody method, however, was too insensitive for use without culture.
两个州公共卫生实验室对用于检测博德特氏菌属的培养和荧光抗体方法进行了评估。如果在采集当天开始培养，接种了现场样本的雷根 - 洛氏琼脂培养基平板最为有用。对于运输并在半强度雷根 - 洛氏琼脂中增菌的鼻咽标本进行传代培养时，雷根 - 洛氏培养基和博 - 金氏培养基效果相当。当两种培养基并行使用时可达到最大灵敏度。鼻咽标本的荧光抗体染色涂片检测百日咳博德特氏菌比检测副百日咳博德特氏菌更敏感。然而，荧光抗体方法如果不结合培养则灵敏度太低，无法使用。
