De Buysscher E V, Breitschwerdt E B, MacLachlan N J
Department of Microbiology, Pathology and Parasitology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Vet Immunol Immunopathol. 1988 Dec;20(1):41-52. doi: 10.1016/0165-2427(88)90024-4.
Immunoglobulin isotypes in serum and intestinal secretions of Basenji dogs with chronic diarrhea, asymptomatic Basenji dogs, and healthy control dogs were quantitated and their molecular sizes characterized to detect alpha-chain, gamma-chain, or mu-chain fragments. Quantitation of immunoglobulin isotypes in serum showed that affected Basenjis have significantly elevated serum IgA values as compared to asymptomatic Basenjis and normal control dogs. However, IgA concentrations in intestinal wash fluids were not significantly different for the three groups. Immunoelectrophoresis (IEP) and polyacrylamide gel electrophoresis (PAGE) demonstrated that virtually all IgA was in the dimeric form. Using IEP and immunoselection, we were unable to detect evidence for the presence of alpha-chain or other heavy-(H)-chain fragments. Hyperimmune serum obtained from rabbits immunized with serum or a globulin fraction of affected Basenjis also failed to detect H-chain fragments. The results of this study indicate that immunoproliferative enteropathy of Basenjis resembles closely the nonsecretory form of human immunoproliferative small intestinal disease (IPSID).
对患有慢性腹泻的巴仙吉犬、无症状巴仙吉犬和健康对照犬的血清及肠道分泌物中的免疫球蛋白同种型进行定量，并对其分子大小进行表征，以检测α链、γ链或μ链片段。血清中免疫球蛋白同种型的定量结果显示，与无症状巴仙吉犬和正常对照犬相比，患病巴仙吉犬的血清IgA值显著升高。然而，三组犬的肠道冲洗液中IgA浓度并无显著差异。免疫电泳（IEP）和聚丙烯酰胺凝胶电泳（PAGE）表明，几乎所有的IgA均为二聚体形式。通过IEP和免疫选择，我们未能检测到α链或其他重链（H链）片段存在的证据。用患病巴仙吉犬的血清或球蛋白组分免疫兔子获得的超免疫血清也未能检测到H链片段。本研究结果表明，巴仙吉犬的免疫增殖性肠病与人类免疫增殖性小肠疾病（IPSID）的非分泌型极为相似。
