Department of Disinfection and Pest Control, Wuhan Centers for Disease Prevention and Control, Wuhan, Hubei, China.
Department of Infectious Disease Prevention, Hubei Center for Disease Control and Prevention, Wuhan, Hubei, China.
PLoS One. 2019 Sep 6;14(9):e0221665. doi: 10.1371/journal.pone.0221665. eCollection 2019.
The ATP bioluminescence method has been increasingly employed as a rapid, on-site detection method in nosocomial infections control. In this study, we used a paired design of monitoring methods, the colony counting method (C) and the ATP bioluminescence method, to evaluate environmental surfaces after disinfection. The ATP bioluminescence method included three detector types (B, P, and N). Every surface after disinfection was performed by combining two types of monitoring methods or detectors. There was no statistically significant difference in theATP content per surface siteamong samples from intensive care units (ICUs)and internal medicine wards using B (p = 0.435) and P (p = 0.260). According to the Spearman's rank correlation coefficients, with the exception of the correlation between the ATP content values detected by B and P, the correlation between the values generated by the remaining methods/detectors was weak or lacking, whereasthe differences between the detectors were statistically significant. Therefore, there are differences between the ATP bioluminescence method and the colony counting method, also between different detectors.
ATP 生物发光法已被越来越多地应用于医院感染控制中的快速现场检测方法。在本研究中，我们使用配对监测方法（菌落计数法（C）和 ATP 生物发光法）来评估消毒后的环境表面。ATP 生物发光法包括三种探测器类型（B、P 和 N）。每次消毒后，我们将两种监测方法或探测器组合使用于每个表面样本。在 ICU 和内科病房中，使用 B（p = 0.435）和 P（p = 0.260）检测的样本中，每个表面位点的 ATP 含量无统计学差异。根据 Spearman 等级相关系数，除了 B 和 P 检测到的 ATP 值之间的相关性外，其余方法/探测器之间的值相关性较弱或不存在，而探测器之间的差异具有统计学意义。因此，ATP 生物发光法与菌落计数法之间存在差异，不同探测器之间也存在差异。
