Research Center for Analytical Sciences, Department of Chemistry, College of Sciences , Northeastern University , Box 332, Shenyang 110819 , China.
ACS Appl Mater Interfaces. 2019 Oct 2;11(39):36286-36295. doi: 10.1021/acsami.9b13718. Epub 2019 Sep 19.
A novel dimercaptosuccinic acid-functionalized mesoporous alumina (DMSA-MA) is synthesized by the dicarboxylic acid groups of dimercaptosuccinic acid molecules coordinating to the Al ions located in the mesostructure. The as-prepared DMSA-MA composites possess a large surface area of 91.17 m/g as well as a uniform pore size and a high pore volume of 17.22 nm and 0.23 cm/g, respectively. DMSA coating of mesostructures significantly enhanced their selectivity for glycoprotein adsorption through a powerful hydrophilic binding force, and the maximum adsorption capacity of immunoglobulin G (IgG) can reach 2298.6 mg g. The captured IgG could be lightly stripped from the DMSA-MA composites with an elution rate of 98.3% by using 0.5 wt % CTAB solution as the elution reagent. DMSA-MA is further employed as a sorbent for the enrichment of IgG heavy chain and light chain from human serum sample. SDS-PAGE assay results showed the obtained IgG with high purity compared to that of the standard solution of IgG.
一种新型的二巯基丁二酸功能化介孔氧化铝（DMSA-MA）通过二巯基丁二酸分子中的羧基与位于介孔结构中的 Al 离子配位合成。所制备的 DMSA-MA 复合材料具有 91.17 m/g 的大表面积以及均匀的孔径和高孔体积，分别为 17.22nm 和 0.23cm/g。介孔结构的 DMSA 涂层通过强大的亲水力显著提高了其对糖蛋白吸附的选择性，免疫球蛋白 G（IgG）的最大吸附容量可达 2298.6mg/g。使用 0.5wt%CTAB 溶液作为洗脱试剂，可将 DMSA-MA 复合材料上捕获的 IgG 以 98.3%的洗脱率轻轻洗脱。DMSA-MA 进一步用作从人血清样品中富集 IgG 重链和轻链的吸附剂。SDS-PAGE 分析结果表明，与 IgG 标准溶液相比，所获得的 IgG 具有较高的纯度。
