Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, China.
Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, Harbin Normal University, Harbin, 150025, China.
Mikrochim Acta. 2022 Jan 3;189(1):43. doi: 10.1007/s00604-021-05155-8.
Metal oxide-based macroporous ordered double affinity molecularly imprinted polymers (D-MIPs) were developed as solid phase extraction (SPE) adsorbents for the specific identification of ovalbumin (OVA) under physiological pH conditions prior to ultraviolet visible (UV-vis) spectrophotometric detection. Herein, macroporous alumina (MA) was used as a matrix; dimercaptosuccinic acid (DMSA) and 3-aminophenylboric acid (APBA) were employed as dual-functional monomers; APBA is a self-polymerizing monomer. The effects of synthesis conditions, SPE conditions as well as selectivity, reproducibility, and reusability were studied. The co-modification of DMSA and boronate affinity renders the adsorbent exhibiting a high adsorption capacity (114.4 mg g) and short equilibrium time (30 min). The surface imprinting technology causes the adsorbent to have high selectivity towards OVA. The OVA recovery range is 91.1-99.6%. This study provides a promising method for the enrichment of OVA and other cis-diol-containing analytes in complex biological samples. A novel metal oxide-based macroporous ordered nanoparticle with a combination of DMSA and boronate affinity was successfully prepared for specific separation and enrichment of glycoprotein from complex biological samples.
基于金属氧化物的大孔有序双亲和分子印迹聚合物（D-MIPs）被开发为固相萃取（SPE）吸附剂，用于在生理 pH 条件下对卵清蛋白（OVA）进行特定识别，然后进行紫外可见（UV-vis）分光光度检测。在此，大孔氧化铝（MA）被用作基质；二巯基丁二酸（DMSA）和 3-氨苯基硼酸（APBA）被用作双功能单体；APBA 是一种自聚合单体。研究了合成条件、SPE 条件以及选择性、重现性和可重复性的影响。DMSA 和硼酸亲和的共修饰使吸附剂表现出高吸附容量（114.4mg g）和短平衡时间（30min）。表面印迹技术使吸附剂对 OVA 具有高选择性。OVA 的回收率范围为 91.1-99.6%。本研究为在复杂生物样品中富集 OVA 和其他含有顺-二醇的分析物提供了一种有前途的方法。成功制备了一种新型基于金属氧化物的大孔有序纳米颗粒，结合了 DMSA 和硼酸亲和性，用于从复杂生物样品中特异性分离和富集糖蛋白。
