Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Environ Int. 2019 Nov;132:105119. doi: 10.1016/j.envint.2019.105119. Epub 2019 Sep 3.
Bike-sharing as a common public transportation has been booming in China in recent years. Previous studies showed that the surfaces of public transport can act as reservoirs of antimicrobial-resistant (AR) bacteria, but AR bacterial contamination of shared bikes has not been investigated. Otherwise, the AR-Enterobacteriaceae is considered as a global health threat for humans. Herein, we aimed to investigate the prevalence of AR Enterobacteriaceae on shared bikes and examine correlations between AR Enterobacteriaceae from shared bikes and public buildings around Metro stations in Beijing. We collected 2117 samples from shared bikes at 240 Metro stations in Beijing. A total of 444 non-duplicate Enterobacteriaceae were isolated from 418 samples at 166 stations. The isolates exhibited low rates of resistance (0.5%-6.3%) to all antimicrobial agents except sulfamethoxazole-trimethoprim (31.5%). Three ceftazidime-resistant E. coli isolates were positive for bla and two of them were positive for carbapenemase-producing gene bla. Multivariable logistic regression model revealed that variable "secondary/tertiary non-profit hospital nearby" was significantly (p < 0.05) associated with isolation of AR Enterobacteriaceae from the shared bikes around the Metro stations. Low AR rates of Enterobacteriaceae observed in this study suggested the risk of dissemination of AR-Enterobacteriaceae via shared bikes is limited. However, we identified hospitals as a risk factor for the dissemination of AR Enterobacteriaceae among shared bike users. More attention should be paid to both comprehensive hygiene managements in the surrounding environment of hospitals and the increasing of public awareness on the personal hygienic habits.
共享单车作为一种常见的公共交通工具，近年来在中国迅速发展。先前的研究表明，公共交通工具的表面可以作为抗微生物药物耐药（AMR）细菌的储库，但共享单车的 AMR 细菌污染尚未得到调查。此外，肠杆菌科 AMR 被认为是对人类的全球健康威胁。在此，我们旨在调查共享单车中肠杆菌科 AMR 的流行情况，并研究共享单车和北京地铁站周围公共建筑中的肠杆菌科 AMR 之间的相关性。我们在北京 240 个地铁站的 2117 辆共享单车上采集了样本。从 166 个车站的 418 个样本中分离出 444 株非重复肠杆菌科。除磺胺甲恶唑-甲氧苄啶（31.5%）外，这些分离株对所有抗菌药物的耐药率均较低（0.5%-6.3%）。3 株头孢他啶耐药大肠杆菌分离株 bla 阳性，其中 2 株 bla 阳性。多变量逻辑回归模型显示，“附近二级/三级非营利性医院”变量与地铁周围共享单车分离肠杆菌科 AMR 显著相关（p < 0.05）。本研究中观察到的肠杆菌科 AMR 率较低表明，通过共享单车传播 AMR-肠杆菌科的风险有限。然而，我们发现医院是共享单车使用者中肠杆菌科 AMR 传播的危险因素。应更加重视医院周边环境的全面卫生管理以及提高公众对个人卫生习惯的认识。
