Chen Qiyan, Zou Zhiyu, Cai Chang, Li Hui, Wang Yang, Lei Lei, Shao Bing
Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Antibiotics (Basel). 2022 Jul 30;11(8):1030. doi: 10.3390/antibiotics11081030.
Shared bikes as a public transport provide convenience for short-distance travel. Whilst they also act as a potential vector for antimicrobial resistant (AR) bacteria and antimicrobial resistance genes (ARGs). However, the understanding of the whole genome sequence of AR strains and ARGs-carrying plasmids collected from shared bikes is still lacking. Here, we used the HiSeq platform to sequence and analyze 24 Escherichia coli isolated from shared bikes around Metro Stations in Beijing. The isolates from shared bikes showed 14 STs and various genotypes. Two blaNDM-5 and blaCTX-M-199-producing ST167 E. coli have 16 resistance genes, four plasmid types and show >95% of similarities in core genomes compared with the ST167 E. coli strains from different origins. The blaNDM-5- or blaCTX-M-199-carrying plasmids sequencing by Nanopore were compared to plasmids with blaNDM-5- or blaCTX-M-199 originated from humans and animals. These two ST167 E. coli show high similarities in core genomes and the plasmid profiles with strains from hospital inpatients and farm animals. Our study indicated that ST167 E. coli is retained in diverse environments and carried with various plasmids. The analysis of strains such as ST167 can provide useful information for preventing or controlling the spread of AR bacteria between animals, humans and environments.
共享单车作为一种公共交通工具，为短途出行提供了便利。然而，它们也可能成为耐抗菌药物（AR）细菌和抗菌耐药基因（ARGs）的传播媒介。然而，目前仍缺乏对从共享单车上收集的AR菌株和携带ARGs的质粒的全基因组序列的了解。在此，我们使用HiSeq平台对从北京地铁站周边共享单车上分离出的24株大肠杆菌进行测序和分析。从共享单车上分离出的菌株显示出14种序列类型（STs）和多种基因型。两株产blaNDM-5和blaCTX-M-199的ST167大肠杆菌有16个耐药基因、四种质粒类型，与来自不同来源的ST167大肠杆菌菌株相比，其核心基因组显示出>95%的相似性。通过纳米孔测序的携带blaNDM-5或blaCTX-M-199的质粒与源自人类和动物的携带blaNDM-5或blaCTX-M-199的质粒进行了比较。这两株ST167大肠杆菌在核心基因组和质粒图谱上与医院住院患者和农场动物的菌株显示出高度相似性。我们的研究表明，ST167大肠杆菌在多种环境中留存，并携带多种质粒。对ST167等菌株的分析可为预防或控制AR细菌在动物、人类和环境之间的传播提供有用信息。
