Institute of Molecular Biology (IMB), Ackermannweg 4, 55128, Mainz, Germany.
Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIEHS, NIH, DHHS, Research Triangle Park, NC, 27709, USA.
Curr Genet. 2020 Apr;66(2):299-302. doi: 10.1007/s00294-019-01023-4. Epub 2019 Sep 6.
Three major eukaryotic DNA polymerases, Polymerases α, δ, and ε (Pols α, δ, and ε), perform the fundamental process of DNA synthesis at the replication fork both accurately and efficiently. In trying to understand the necessity and flexibility of the polymerase usage, we recently reported that budding yeast cells lacking Pol ε exonuclease and polymerase domains (pol2-16) survive, but have severe growth defects, checkpoint activation, increased level of dNTP pools as well as significant increase in the mutation rates. Herein, we suggest new opportunities to distinguish the roles of Pol ε from those of two other eukaryotic B-family DNA polymerases, Pols δ and ζ.
三种主要的真核生物 DNA 聚合酶，聚合酶α、δ 和 ε（Pols α、δ 和 ε），在复制叉处高效且准确地执行 DNA 合成的基本过程。为了了解聚合酶使用的必要性和灵活性，我们最近报道了缺失 Pol ε 核酸外切酶和聚合酶结构域（pol2-16）的酿酒酵母细胞能够存活，但生长缺陷严重，检查点激活，dNTP 池水平升高，突变率显著增加。在此，我们提出了一种新的方法来区分 Pol ε 与另外两种真核 B 族 DNA 聚合酶 Pols δ 和 ζ 的作用。
