Department of Geriatrics, Tongji Hospital, Tongji University, School of Medicine, Shanghai, China (mainland).
Department of Interventional and Vascular Surgery, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, China (mainland).
Med Sci Monit. 2019 Sep 7;25:6719-6726. doi: 10.12659/MSM.915532.
BACKGROUND Bariatric procedures such as left gastric artery ligation (LGAL) and sleeve gastrectomy (SG) have emerged as important procedures for treating morbid obesity. In this study, we compared the effects of LGAL vs. SG on obesity-induced adipose tissue macrophage infiltration and inflammation in diet-induced obese rats. MATERIAL AND METHODS Sprague-Dawley (SD) rats were fed a high-fat diet (HFD) for 16 weeks to induce obesity. SG, GLAL, or corresponding sham surgeries were performed in anesthetized rats. Inflammatory factor expression in serum and epididymal and retroperitoneal adipose tissues were analyzed 4 weeks after surgery. Macrophage infiltration and phenotype transformation were also assessed with Western blot analysis and immunofluorescence. RESULTS Both LGAL and SG strongly attenuated high-fat diet (HFD)-induced fat accumulation in retroperitoneal and epididymal tissues. The expressions of inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1 were downregulated after LGAL and after SG by promoting activation of M2 macrophages, despite continued exposure to HFD. Furthermore, both LGAL and SG resulted in increased macrophage infiltration, but did not contribute to phenotype transformation of macrophages to M1. CONCLUSIONS LGAL and SG both reduced fat accumulation caused by HFD feeding. Therapies designed to ameliorate the inflammatory response by promoting activation of M2 macrophages may be valuable.
减重手术，如胃左动脉结扎术（LGAL）和袖状胃切除术（SG），已成为治疗病态肥胖的重要手段。本研究旨在比较 LGAL 与 SG 对肥胖诱导的脂肪组织巨噬细胞浸润和炎症的影响。
SD 大鼠给予高脂肪饮食（HFD）喂养 16 周以诱导肥胖。在麻醉大鼠中进行 SG、LGAL 或相应的假手术。术后 4 周分析血清和附睾及腹膜后脂肪组织中炎症因子的表达。通过 Western blot 分析和免疫荧光评估巨噬细胞浸润和表型转化。
LGAL 和 SG 均可强烈减轻 HFD 诱导的腹膜后和附睾脂肪堆积。尽管继续暴露于 HFD，LGAL 和 SG 后促 M2 巨噬细胞活化可下调肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6 和单核细胞趋化蛋白（MCP）-1 等炎症因子的表达。此外，LGAL 和 SG 均导致巨噬细胞浸润增加，但对巨噬细胞向 M1 表型转化没有贡献。
LGAL 和 SG 均可减轻 HFD 喂养引起的脂肪堆积。通过促进 M2 巨噬细胞活化来改善炎症反应的治疗方法可能具有价值。
