Tianjin Medical University, No. 22 Meteorological Observatory Road, Heping District, Tianjin, China; Tianjin Central Hospital of Gynecology Obstetrics, No. 156 Nankai San Ma Road, Nankai District, Tianjin, China.
Jining No.1 People's Hospital, Shandong, No. 6 Health Road, Rencheng District, Jining, China.
Virus Res. 2019 Nov;273:197740. doi: 10.1016/j.virusres.2019.197740. Epub 2019 Sep 4.
The aim of this study is to investigate distribution of human papillomavirus (HPV) 16 variants in screening healthy women and the potential association between HPV 16 variants and progression of cervical lesions. For this study a total of 2000 healthy women in Tianjin urban area and 212 patients who were HPV 16 positive and underwent colposcopy were analyzed for HPV 16 variants by pyrosequencing. The results show that the HPV 16 was the most prevalent genotype in Tianjin healthy women and five HPV 16 variant types were detected. The HPV 16 variants were determined by sequencing parital E6 region and the detected variants were European prototype E-T350 (E-p), E-G350, E-C109 G, Asian (As) and Asian-American (AA), among which the E-p variant was the most prevalent (82.76%) followed by As variant. Interestingly, in patients with suspected cervical lesions the most prevalent variant was As variant (54.9%) by increasing significance with severity of cervical diseases (OR 4.337; 95% CI 1.248-15.067; P = 0.021), and followed by HPV 16 E-p variant while E-G350 variant only appeared in HSIL and cervical cancer. Our results show that HPV 16 E-p variant was more prevalent than As in Tianjin healthy screening women while As variant was the most frequently type in HSIL and cervical cancer. It is suggested that the mutation of HPV 16 Asian variants, comparing with HPV 16 E-p variants, might contribute to the transformation from HPV 16 persistent infection to cervical cancer.
本研究旨在调查人乳头瘤病毒（HPV）16 变异株在筛查健康女性中的分布，以及 HPV 16 变异株与宫颈病变进展之间的潜在关联。为此，对天津市城区 2000 名健康女性和 212 名 HPV 16 阳性并接受阴道镜检查的患者进行了 HPV 16 变异株的焦磷酸测序分析。结果表明，HPV 16 是天津健康女性中最常见的基因型，共检测到 5 种 HPV 16 变异型。通过对 E6 区部分序列的测序确定了 HPV 16 变异株，检测到的变异株包括欧洲原型 E-T350（E-p）、E-G350、E-C109G、亚洲（As）和亚裔美国人（AA），其中 E-p 变异株最为常见（82.76%），其次是 As 变异株。有趣的是，在疑似宫颈病变患者中，随着宫颈疾病严重程度的增加，最常见的变异株是 As 变异株（54.9%）（OR 4.337；95%CI 1.248-15.067；P=0.021），其次是 HPV 16 E-p 变异株，而 E-G350 变异株仅出现在 HSIL 和宫颈癌中。本研究结果表明，HPV 16 E-p 变异株在天津健康筛查女性中比 As 变异株更为常见，而 As 变异株是 HSIL 和宫颈癌中最常见的类型。提示 HPV 16 亚洲变异株的突变，与 HPV 16 E-p 变异株相比，可能有助于 HPV 16 持续感染向宫颈癌的转化。
