Key Laboratory for Biorheological Science and Technology of Ministry of Education (Chongqing University), Chongqing University Cancer Hospital & Chongqing Cancer Institute & Chongqing Cancer Hospital, Chongqing 400044, China.
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Oxid Med Cell Longev. 2020 Apr 19;2020:3483519. doi: 10.1155/2020/3483519. eCollection 2020.
In this study, flavonoids in lemon seeds (FLS) were used to assess its improvement on the oxidative damage of human embryonic kidney 293T cells (HEK 293T cells) induced by HO. experiments showed that the survival rates of HEK 293T cells treated with different flavonoid concentrations (50 g/mL, 100 g/mL, and 150 g/mL) exceeded 95%, indicating no significant toxic effect. Compared with the normal group, HO (0.3 mmol/L) resulted significantly in oxidative stress injury of HEK 293T cells. The survival rate of the damaged cells increased after treatment with flavonoids, and the survival rate of cells treated with a high concentration (150 g/mL) of flavonoids was 76.2%. Flavonoids also effectively inhibited HO-induced apoptosis. At the same time, flavonoid treatment significantly reduced the malondialdehyde content in cells and increased the levels of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px). Quantitative polymerase chain reaction (qPCR) and Western blot analysis also suggested that FLS upregulated mRNA and protein expressions of CAT, SOD (SOD1, SOD2), GSH (GSH1), and GSH-Px in HO-induced oxidative damage of HEK 293T cells. The high-performance liquid chromatography analysis demonstrated that FLS contained six compounds, including gallocatechin, caffeic acid, epicatechin, vitexin, quercetin, and hesperidin. FLS were proven to have a good antioxidant capacity and improve significantly the oxidative damage of HEK 293T cells induced by HO. The biological activity value warrants investigation in additional studies.
在这项研究中，利用柠檬籽中的类黄酮（FLS）来评估其对 HO 诱导的人胚肾 293T 细胞（HEK 293T 细胞）氧化损伤的改善作用。实验表明，不同黄酮浓度（50μg/ml、100μg/ml 和 150μg/ml）处理的 HEK 293T 细胞存活率均超过 95%，表明无明显毒性作用。与正常组相比，HO（0.3mmol/L）显著导致 HEK 293T 细胞氧化应激损伤。黄酮处理后，损伤细胞的存活率增加，高浓度（150μg/ml）黄酮处理细胞的存活率为 76.2%。黄酮还能有效抑制 HO 诱导的细胞凋亡。同时，黄酮处理显著降低了细胞内丙二醛含量，提高了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的水平。定量聚合酶链反应（qPCR）和 Western blot 分析也表明，FLS 上调了 HO 诱导的 HEK 293T 细胞氧化损伤中 CAT、SOD（SOD1、SOD2）、GSH（GSH1）和 GSH-Px 的 mRNA 和蛋白表达。高效液相色谱分析表明，FLS 含有六种化合物，包括没食子儿茶素、咖啡酸、表儿茶素、牡荆素、槲皮素和橙皮苷。FLS 具有良好的抗氧化能力，能显著改善 HO 诱导的 HEK 293T 细胞氧化损伤。其生物活性值有待进一步研究。
