Graduate student, Graduate Program in Dentistry, School of Life Sciences, Pontifícia, Universidade Católica do Paraná, Rua Imaculada Conceição 1155, Curitiba, PR, 80215-901, Brazil.
Undergraduate student, Dentistry, School of Life Sciences, Pontifícia Universidade, Católica do Paraná, Rua Imaculada Conceição 1155, Curitiba, PR, 80215-901, Brazil.
Drug Alcohol Depend. 2019 Nov 1;204:107510. doi: 10.1016/j.drugalcdep.2019.06.013. Epub 2019 Aug 29.
Alcohol and substances found in tobacco may alter salivary flow and amount of saliva proteins. This study aimed to compare salivary proteins between alcohol dependent smokers and controls.
This is a case-control study with men older than 18 years of age, matched by age. The alcohol-dependent group was composed by heavy smokers and alcohol consumers. Unstimulated whole saliva was collected from all subjects. Analysis of digested peptides was performed in mass spectrometer. Data were processed using ProteinLynx GlobalServer software. Results were obtained by searching theHomo sapiens database from the UniProt catalog. The search tool IBI-IMIM was used to identify candidate proteins for biomarkers.
Alcohol-dependent and control groups were composed of nine participants each, with mean age of 36.89 ± 2.57 and 35.78 ± 1.64 years, respectively. 404 salivary proteins were found in both groups; 282 in the alcohol-dependent. Among the 96 proteins presented in both groups, 32 were up-regulated in the alcohol dependents (i.e. "Hemoglobin subunit beta" and "Forkhead box protein P2" were up-regulated at least 10-fold), 23 were down-regulated (i.e. "Statherin" and "RNA-binding protein 25" were down-regulated at least 10-fold), and 41 presented similar expression in both groups. 71 proteins were candidates for biomarkers of disorders 58 presented in alcohol dependents' saliva. The most common disorders were neoplasms, genetic, cardiovascular, metabolic and glandular diseases.
Salivary protein profile undergoes strong changes in alcohol and tobacco dependents. 34% of salivary proteins present in alcohol and tobacco dependents were present in controls; 14.5% of them were expressed in similar quantity.
酒精和烟草中的物质可能会改变唾液流量和唾液蛋白的含量。本研究旨在比较酒精依赖吸烟者和对照组之间的唾液蛋白。
这是一项病例对照研究，纳入年龄大于 18 岁的男性，并按年龄匹配。酒精依赖组由重度吸烟者和饮酒者组成。所有受试者均采集非刺激性全唾液。使用质谱仪对消化后的肽进行分析。使用 ProteinLynx GlobalServer 软件处理数据。结果通过搜索 UniProt 目录中的 Homo sapiens 数据库获得。使用 IBI-IMIM 搜索工具来识别候选生物标志物蛋白。
酒精依赖组和对照组各包含 9 名参与者，平均年龄分别为 36.89±2.57 岁和 35.78±1.64 岁。两组均发现 404 种唾液蛋白，其中 282 种在酒精依赖组中发现。在两组共有的 96 种蛋白中，32 种在酒精依赖组中上调（即“血红蛋白亚基β”和“叉头框蛋白 P2”上调至少 10 倍），23 种下调（即“Statherin”和“RNA 结合蛋白 25”下调至少 10 倍），41 种在两组中的表达相似。71 种蛋白候选为疾病生物标志物，其中 58 种在酒精依赖者的唾液中存在。最常见的疾病是肿瘤、遗传、心血管、代谢和腺体疾病。
酒精和烟草依赖者的唾液蛋白谱发生了强烈变化。在酒精和烟草依赖者中存在的 34%的唾液蛋白在对照组中存在；其中 14.5%以相似的量表达。
