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生物玻璃/PLGA 联合光生物调节:对颅骨骨缺损实验模型愈合过程的影响。

Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect.

机构信息

Federal University of São Paulo (UNIFESP), Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil.

University Center of the Guaxupé Educational Foundation (UNIFEG), Avenida Dona Floriana, Guaxupé, MG, 37800000, Brazil.

出版信息

J Mater Sci Mater Med. 2019 Sep 7;30(9):105. doi: 10.1007/s10856-019-6307-x.

Abstract

Bioactive glasses (BG) are known for their ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not enough to induce bone consolidation. Thus, the enrichment of BG with polymers such as Poly (D, L-lactic-co-glycolic) acid (PLGA) and associated to photobiomodulation (PBM) may be a promising strategy to promote bone tissue healing. The aim of the present study was to investigate the in vivo performance of PLGA supplemented BG, associated to PBM therapy, using an experimental model of cranial bone defect in rats. Rats were distributed in 4 different groups (Bioglass, Bioglass/PBM, Bioglas/PLGA and BG/PLGA/PBM). After the surgical procedure to induce cranial bone defects, the pre-set samples were implanted and PBM treatment (low-level laser therapy) started (808 nm, 100 mW, 30 J/cm). After 2 and 6 weeks, animals were euthanized, and the samples were retrieved for the histopathological, histomorphometric, picrosirius red staining and immunohistochemistry analysis. At 2 weeks post-surgery, it was observed granulation tissue and areas of newly formed bone in all experimental groups. At 6 weeks post-surgery, BG/PLGA (with or without PBM) more mature tissue around the biomaterial particles. Furthermore, there was a higher deposition of collagen for BG/PLGA in comparison with BG/PLGA/PBM, at second time-point. Histomorphometric analysis demonstrated higher values of BM.V/TV for BG compared to BG/PLGA (2 weeks post-surgery) and N.Ob/T.Ar for BG/PLGA compared to BG and BG/PBM (6 weeks post-surgery). This current study concluded that the use of BG/PLGA composites, associated or not to PBM, is a promising strategy for bone tissue engineering.

摘要

生物活性玻璃(BG)以其与骨组织结合的能力而闻名。然而,在危急情况下,即使 BG 的成骨特性也可能不足以诱导骨整合。因此,用聚合物如聚(D,L-乳酸-共-乙醇酸)(PLGA)对 BG 进行富集,并与光生物调节(PBM)相结合,可能是促进骨组织愈合的一种有前途的策略。本研究的目的是使用大鼠颅骨缺损实验模型研究 PLGA 补充 BG 与 PBM 治疗联合应用的体内性能。大鼠被分为 4 个不同的组(Bioglass、Bioglass/PBM、Bioglass/PLGA 和 BG/PLGA/PBM)。在颅骨缺损手术完成后,植入预设样本并开始 PBM 治疗(808nm,100mW,30J/cm)。2 和 6 周后,处死动物,取出样本进行组织病理学、组织形态计量学、苦味酸红染色和免疫组织化学分析。手术后 2 周,观察到所有实验组均有肉芽组织和新形成骨的区域。手术后 6 周,BG/PLGA(有或没有 PBM)在生物材料颗粒周围有更成熟的组织。此外,与 BG/PLGA/PBM 相比,BG/PLGA 中有更高的胶原蛋白沉积,在第二个时间点。组织形态计量学分析表明,与 BG/PLGA 相比,BG 的 BM.V/TV 值更高(手术后 2 周),与 BG 和 BG/PBM 相比,BG/PLGA 的 N.Ob/T.Ar 值更高(手术后 6 周)。本研究得出结论,使用 BG/PLGA 复合材料,无论是否与 PBM 联合使用,都是骨组织工程的一种有前途的策略。

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