Birulina Yu G, Petrova I V, Rozenbaum Yu A, Shefer E A, Smagliy L V, Nosarev A V, Gusakova S V
Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
Bull Exp Biol Med. 2019 Aug;167(4):508-511. doi: 10.1007/s10517-019-04561-6. Epub 2019 Sep 7.
The effect of HS on changes in erythrocyte volume was studied by spectrophotometrical and potentiometric methods. It was found that HS donor NaHS (2.5, 10, and 100 μM) induced an increase in erythrocyte volume in heterosmotic media. Activation of Gardos channels with A23187 or ascorbate-phenazine methosulfate system causes erythrocyte shrinkage and hyperpolarization of their membrane, while addition of NaHS restored erythrocyte volume. The decrease in erythrocyte volume upon blockade of Na,K,2Cl cotransporter (bumetanide) or anion exchanger (SITS) was abolished by HS donor NaHS, which attested to an important role of these transporters and chlorine conductivity of the membrane in the maintenance of the homeostasis of blood cells.
通过分光光度法和电位分析法研究了硫化氢（HS）对红细胞体积变化的影响。研究发现，HS供体硫氢化钠（NaHS，浓度分别为2.5、10和100 μM）可使异渗介质中的红细胞体积增加。用A23187或抗坏血酸 - 吩嗪硫酸甲酯系统激活加尔多斯通道会导致红细胞收缩及其膜超极化，而添加NaHS可恢复红细胞体积。HS供体NaHS消除了在阻断钠 - 钾 - 2氯协同转运蛋白（布美他尼）或阴离子交换蛋白（SITS）后红细胞体积的减少，这证明了这些转运蛋白和膜的氯传导性在维持血细胞内环境稳定中起重要作用。
