Cardiovascular and Metabolic Research Unit, Laurentian University, Sudbury, Ontario, Canada.
School of Life Science, Shanxi University, Taiyuan, People's Republic of China.
Am J Physiol Regul Integr Comp Physiol. 2020 Jul 1;319(1):R69-R78. doi: 10.1152/ajpregu.00348.2019. Epub 2020 May 20.
The production of HS and its effect on bioenergetics in mammalian cells may be evolutionarily preserved. Erythrocytes of birds, but not those of mammals, have a nucleus and mitochondria. In the present study, we report the endogenous production of HS in chicken erythrocytes, which was mainly catalyzed by 3-mercaptopyruvate sulfur transferase (MST). ATP content of erythrocytes was increased by MST-generated endogenous HS under normoxic, but not hypoxic, conditions. NaHS, a HS salt, increased ATP content under normoxic, but not hypoxic, conditions. ATP contents in the absence or presence of NaHS were eliminated by different inhibitors for mitochondrial electron transport chain in chicken erythrocytes. Succinate and glutamine, but not glucose, increased ATP content. NaHS treatment similarly increased ATP content in the presence of glucose, glutamine, or succinate, respectively. Furthermore, the expression and activity of sulfide:quinone oxidoreductase were enhanced by NaHS. The structural integrity of chicken erythrocytes was largely maintained during 2-wk NaHS treatment in vitro, whereas most of the erythrocytes without NaHS treatment were lysed. In conclusion, HS may regulate cellular bioenergetics as well as cell survival of chicken erythrocytes, in which the functionality of the electron transport chain is involved. HS may have different regulatory roles and mechanisms in bioenergetics of mammalian and bird cells.
HS 的产生及其对哺乳动物细胞生物能量学的影响可能是进化上保守的。鸟类的红细胞，而不是哺乳动物的红细胞，具有细胞核和线粒体。在本研究中，我们报告了鸡红细胞内源性 HS 的产生，主要由 3-巯基丙酮酸硫转移酶（MST）催化。在常氧而非缺氧条件下，MST 产生的内源性 HS 增加了红细胞的 ATP 含量。HS 盐 NaHS 在常氧而非缺氧条件下增加了 ATP 含量。鸡红细胞中线粒体电子传递链的不同抑制剂消除了无 NaHS 或有 NaHS 存在时的 ATP 含量。琥珀酸盐和谷氨酰胺，但不是葡萄糖，增加了 ATP 含量。NaHS 处理分别在存在葡萄糖、谷氨酰胺或琥珀酸盐的情况下同样增加了 ATP 含量。此外，NaHS 增强了硫代醌氧化还原酶的表达和活性。在体外 2 周的 NaHS 处理过程中，鸡红细胞的结构完整性在很大程度上得以维持，而没有 NaHS 处理的大多数红细胞都发生了裂解。总之，HS 可能调节鸡红细胞的细胞生物能量学和细胞存活，其中涉及电子传递链的功能。HS 在哺乳动物和鸟类细胞的生物能量学中可能具有不同的调节作用和机制。
