Alber D, Moussard C, Toubin M, Henry J C, Ottignon Y, Deschamps J P
Laboratoire de Biochimie Médicale, C.H.R. St Jacques, Besançon, France.
Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):299-304. doi: 10.1002/bms.1200160157.
In order to investigate the contribution of eicosanoids to human oesophageal functions and disorders (gastrooesophageal reflux, GOR and reflux oesophagitis, RO), we have used a selected ion monitoring gas chromatographic/mass spectrometric methodology to quantify the cyclooxygenase and lipoxygenase products biosynthesized in vitro by endoscopic mucosal biopsy specimens. Prostaglandins (PGs) were quantified as MEMOTMS derivatives and HETEs, as hydrogenated methyl ester of tert-butyldimethylsilyl) ether derivatives. PGE2, PGF2 alpha appeared as the major prostanoids, whereas 12HETE seemed to be the major lipoxygenase product. In the case of GOR or RO, biosynthesis of PGE2 was dramatically increased, while no change could be detected for 12HETE. PGE2 increase seems to be related to inflammatory reaction, in which its exact role remains unclear. Moreover, it cannot be excluded that PGE2 is a side product which might be protective to the oesophageal mucosa.
为了研究类二十烷酸对人类食管功能及疾病（胃食管反流，GOR；反流性食管炎，RO）的作用，我们采用了选择性离子监测气相色谱/质谱分析法，对通过内镜黏膜活检标本体外生物合成的环氧化酶和脂氧合酶产物进行定量分析。前列腺素（PGs）被定量为MEMOTMS衍生物，而羟二十碳四烯酸（HETEs）则为叔丁基二甲基甲硅烷基醚衍生物的氢化甲酯。PGE2、PGF2α似乎是主要的前列腺素，而12-HETE似乎是主要的脂氧合酶产物。在GOR或RO病例中，PGE2的生物合成显著增加，而12-HETE未检测到变化。PGE2的增加似乎与炎症反应有关，其确切作用尚不清楚。此外，不能排除PGE2是一种可能对食管黏膜有保护作用的副产物。
