Pengpumkiat Sumate, Wu Yuanyuan, Sumantakul Saichon, Remcho Vincent T
Department of Chemistry, Oregon State University, 153 Gilbert Hall, Corvallis, OR, 97331, USA.
Anal Sci. 2020 Feb 10;36(2):193-197. doi: 10.2116/analsci.19P267. Epub 2019 Sep 6.
A novel approach to building a membrane-based disposable well-plate, here applied to cyanide detection, is described. Chitosan encapsulated CdTe quantum dots with a maximum emission at 520 nm (CS-QD520) were used as fluorophores. The CS-QD520 nanoparticle was specifically quenched by copper(II), and the quenched CS-QD520 (Cu-CS-QD520) was deposited onto a glass microfiber filter (GF/B). Subsequent introduction of cyanide ion resulted in fluorescence recovery. The "signal-ON" fluorescence linearly correlated to cyanide concentrations in the range of 38.7 to 200 μM with a limit of detection of 11.6 μM. The assay was incorporated into a membrane-based well-plate format to enhance sample throughput. A three-layer paper/glass microfiber well plate design was cut using a laser cutter and assembled using a polycaprolactone (PCL) as a bonding agent in a low-cost laminator. The experimental conditions were optimized and applied to detect cyanide in drinking water with rapid, high-throughput, low-cost analysis.
本文描述了一种构建基于膜的一次性微孔板的新方法，该方法在此用于氰化物检测。壳聚糖包裹的最大发射波长为520 nm的碲化镉量子点（CS-QD520）用作荧光团。CS-QD520纳米颗粒被铜（II）特异性猝灭，猝灭后的CS-QD520（Cu-CS-QD520）沉积在玻璃微纤维滤膜（GF/B）上。随后引入氰离子导致荧光恢复。“信号开启”荧光与38.7至200 μM范围内的氰化物浓度呈线性相关，检测限为11.6 μM。该检测方法被整合到基于膜的微孔板形式中以提高样品通量。使用激光切割机切割三层纸/玻璃微纤维微孔板设计，并在低成本层压机中使用聚己内酯（PCL）作为粘合剂进行组装。对实验条件进行了优化，并将其应用于饮用水中氰化物的快速、高通量、低成本分析检测。
