Nishikawa S, Fujiwara K, Kitamura H
Department of Oral Histology, Kanagawa Dental College, Japan.
Eur J Cell Biol. 1988 Dec;47(2):222-32.
The localization of actin, myosin, tropomyosin, alpha-actinin, vinculin, and desmoplakin I/II was visualized by immunofluorescence microscopy. Antibodies against myosin, tropomyosin, and alpha-actinin and rhodamine-phalloidin labeled strongly the proximal and distal terminal webs which ultrastructurally consist of dense microfilament bundles. In the distal terminal web, the staining by these reagents occurred mostly perpendicular to the long axis of the incisor. Antivinculin stained the general area where the distal terminal web is located in the ameloblast. Anti-desmoplakin I/II labeled the junctional area associated with the proximal and distal terminal webs. The anti-desmoplakin staining was stronger along the cell border perpendicular to the long axis of the incisor. Comparison of the rhodamine-phalloidin staining pattern of the distal terminal web and the enamel secretion pattern by ameloblasts revealed that a change in the distal terminal web staining pattern preceded a change in the secretion pattern. These observations suggest that the cytoskeletal organization in the ameloblast is involved in the formation of the enamel matrix pattern in the rat incisor.
通过免疫荧光显微镜观察肌动蛋白、肌球蛋白、原肌球蛋白、α-辅肌动蛋白、纽蛋白和桥粒斑蛋白I/II的定位。抗肌球蛋白、原肌球蛋白和α-辅肌动蛋白抗体以及罗丹明-鬼笔环肽强烈标记近端和远端终末网，超微结构上其由密集的微丝束组成。在远端终末网中，这些试剂的染色大多垂直于切牙的长轴。抗纽蛋白染色标记成釉细胞中远端终末网所在的大致区域。抗桥粒斑蛋白I/II标记与近端和远端终末网相关的连接区域。抗桥粒斑蛋白染色在垂直于切牙长轴的细胞边界处更强。远端终末网的罗丹明-鬼笔环肽染色模式与成釉细胞的釉质分泌模式的比较显示，远端终末网染色模式的变化先于分泌模式的变化。这些观察结果表明，成釉细胞中的细胞骨架组织参与了大鼠切牙釉质基质模式的形成。
