Wang Qingmei, Xu Qianzi, Wei Anyi, Chen Shishuo, Zhang Chong, Zeng Linghui
School of Medicine, Zhejiang University City College, Hangzhou 310015, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2019 May 25;48(3):296-302. doi: 10.3785/j.issn.1008-9292.2019.06.10.
To investigate the effects of high dose vitamin C (VC) on proliferation of breast cancer cells and to explore its mechanisms.
Human breast cancer cells Bcap37 and MDA-MB-453 were treated with VC at low dose (0.01 mmol/L), medium dose (0.10 mmol/L) and high dose (2.00 mmol/L). Cell proliferation was determined with CCK-8 assay, protein expression was evaluated by Western blot, and the secretion of lactic acid in tumor cells was detected by colorimetric method. Bcap37 cells were inoculated in nude mice, and tumor baring nude mice were intraperitoneally injected with high VC(4 g/kg, VC group, =5)or normal saline (control group, =5) for 24 d. Tumor weight and body weight were calculated.
experiments demonstrated that high dose VC significantly inhibited cell proliferation in Bcap37 and MDA-MB-453 cells (all <0.01); the expressions of Glut1 and mTOR signaling pathway-related proteins were decreased (all <0.05); and the secretion of lactic acid was also markedly reduced (all <0.05). experiment showed that the tumor weight was decreased in mice treated with high-dose VC as compared with control group (<0.05), but no difference in body weights between two groups was observed.
High dose VC may inhibit proliferation of breast cancer cells both and through reducing glycolysis and protein synthesis.
探讨高剂量维生素C（VC）对乳腺癌细胞增殖的影响并探究其作用机制。
采用低剂量（0.01 mmol/L）、中剂量（0.10 mmol/L）和高剂量（2.00 mmol/L）的VC处理人乳腺癌细胞Bcap37和MDA-MB-453。采用CCK-8法检测细胞增殖，通过蛋白质印迹法评估蛋白表达，并采用比色法检测肿瘤细胞中乳酸的分泌。将Bcap37细胞接种于裸鼠体内，对荷瘤裸鼠腹腔注射高剂量VC（4 g/kg，VC组，n = 5）或生理盐水（对照组，n = 5），持续24天。计算肿瘤重量和体重。
实验表明，高剂量VC显著抑制Bcap37和MDA-MB-453细胞的增殖（均P < 0.01）；葡萄糖转运蛋白1（Glut1）和雷帕霉素靶蛋白（mTOR）信号通路相关蛋白的表达降低（均P < 0.05）；乳酸分泌也明显减少（均P < 0.05）。实验表明，与对照组相比，高剂量VC处理的小鼠肿瘤重量降低（P < 0.05），但两组小鼠体重无差异。
高剂量VC可能通过减少糖酵解和蛋白质合成在体内和体外抑制乳腺癌细胞的增殖。
