锰掺杂硫化锌量子点的小鼠毒性及血清代谢组学研究。
Toxicity and serum metabolomics investigation of Mn-doped ZnS quantum dots in mice.
机构信息
School of Basic Medical Sciences, Henan University, Kaifeng 475004, People's Republic of China.
Henan Provincial Engineering Centre of Tumor Molecular Diagnosis and Therapy & Kaifeng Municipal Key Laboratory of Cell Signal Transduction, Henan University , Kaifeng 475004, People's Republic of China.
出版信息
Int J Nanomedicine. 2019 Aug 6;14:6297-6311. doi: 10.2147/IJN.S212355. eCollection 2019.
PURPOSE
Mn-doped ZnS quantum dots (QDs) with special luminescent properties have been widely researched and applied in various fields. Thus, their release toxicity and security cannot be ignored.
METHODS
In the present study, the toxicity and non-targeted metabolomics of Mn-doped ZnS QDs were investigated after single intravenous injection. Serum metabolites were evaluated based on gas chromatography-mass spectrometry together with multivariate statistical analyses [principal component analysis, partial least squares discriminant analysis, and orthogonal PLS-DA].
RESULTS
The modified metabolites (variable importance in the projection (VIP) >1 and <0.05) revealed that Mn-doped ZnS QDs exposure disturbed glycolysis, tricarboxylic acid cycle, ketoplasia, glutaminolysis, and amino acid and lipid metabolism. The behavior, coefficients of organs, and histological changes were the same as in the control group, and the disturbance of hematology and serum biochemistry was not dose- or time-dependent.
CONCLUSION
Our study provides a general observation regarding the toxicity and potential metabolic responses of mice exposed to Mn-doped ZnS QDs.
目的
具有特殊发光性能的锰掺杂硫化锌量子点（QDs）已被广泛研究和应用于各个领域，因此其释放毒性和安全性不容忽视。
方法
本研究采用单静脉注射法研究了锰掺杂硫化锌 QDs 的毒性和非靶向代谢组学。采用气相色谱-质谱联用技术结合多元统计分析（主成分分析、偏最小二乘判别分析和正交偏最小二乘判别分析）对血清代谢物进行了评估。
结果
修饰后的代谢物（投影重要性（VIP）>1 且<0.05）表明，锰掺杂硫化锌 QDs 暴露会干扰糖酵解、三羧酸循环、酮质体、谷氨酰胺分解和氨基酸及脂质代谢。行为、器官系数和组织学变化与对照组相同，血液学和血清生化的紊乱与剂量或时间无关。
结论
本研究为观察锰掺杂硫化锌 QDs 暴露对小鼠的毒性和潜在代谢反应提供了一个总体观察。
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