Institute for Risk Analysis and Risk Communication, Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
National Institute for Occupational Health, and Hematology and Molecular Medicine Department, University of the Witwatersrand, Johannesburg, South Africa.
Biomed Res Int. 2018 Jul 22;2018:8460910. doi: 10.1155/2018/8460910. eCollection 2018.
To investigate the effect of subacute intravenous administration AgNP (silver nanoparticles, 10 nm) and AuNP (gold nanoparticles, 12.8 nm) and AgNP/AuNP mixture to blood biochemistry, hematology, and platelet coagulation, subacute toxicity study was conducted.
AuNP and AgNP in which their size distribution was not statistically different, mixed or separate, were injected into the caudal vein of male Sprague-Dawley rats for 4 weeks. The rats were allowed to recover for a further 4 weeks in order to examine systemic toxicity expressed in the blood biochemistry and hematology. The dose groups (5 males per group for the administration and 3 males for the recovery) consisted of 7 divisions, i.e., control, AgNP (with a low dose of 10 g/kg/day and a high dose of 100 g/kg/day), AuNP (with a low dose of 10 g/kg/day and a high dose of 100 g/kg/day), and mixed AgNP/AuNP (with a low dose of 10/10 g/kg/day and a high dose of 100/100 g/kg/day).
There were no significant dose-related changes in the hematology and blood biochemical values for the rats. Coagulation time in terms of the active partial thromboplastin time (APTT) and prothrombin time (PT) did not show any significant changes, when compared to the control group.
The subacute injection of AuNP and AgNP or their mixture did not induce any noticeable systemic toxicity.
为了研究亚急性静脉注射 AgNP（银纳米粒子，10nm）和 AuNP（金纳米粒子，12.8nm）以及 AgNP/AuNP 混合物对血液生化、血液学和血小板凝血的影响，进行了亚急性毒性研究。
将大小分布无统计学差异的 AuNP 和 AgNP 混合或单独注射到雄性 Sprague-Dawley 大鼠的尾静脉中，进行 4 周的治疗。为了检查血液生化和血液学中表达的全身毒性，大鼠在进一步的 4 周恢复期内被允许恢复。给药组（每组 5 只雄性用于给药，3 只雄性用于恢复）包括 7 个剂量组，即对照组、AgNP（低剂量 10g/kg/天和高剂量 100g/kg/天）、AuNP（低剂量 10g/kg/天和高剂量 100g/kg/天）和混合 AgNP/AuNP（低剂量 10/10g/kg/天和高剂量 100/100g/kg/天）。
大鼠的血液学和血液生化值没有出现明显的剂量相关性变化。与对照组相比，活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）的凝血时间没有出现任何显著变化。
亚急性注射 AuNP 和 AgNP 或它们的混合物不会引起明显的全身毒性。
