Liu Chengjun, Zhang Zuwang, Qi Dongdong
Thoracic Surgery, University-Town Hospital of Chongqing Medical University, Chongqing 401331, People's Republic of China.
Onco Targets Ther. 2019 Aug 5;12:6181-6189. doi: 10.2147/OTT.S201834. eCollection 2019.
Circular RNAs (circRNAs) have been considered as key regulators of cancer biology. However, the functional role of hsa_circ_0023404 in non-small cell lung cancer (NSCLC) and its regulatory mechanism are still almost unknown.
The expression of hsa_circ_0023404, miR-217 and zinc finger E-box-binding homeobox 1 (ZEB1) was evaluated by quantitative real-time polymerase chain reaction. The role of hsa_circ_0023404 in NSCLC progression was determined using cell count kit-8 assay, transwell migration and invasion assay. Luciferase reporter assay was performed to assess the interaction of hsa_circ_0023404, miR-217 and ZEB1 in NSCLC cells.
The expression of hsa_circ_0023404 was upregulated in NSCLC tissues, as well as in NSCLC cell lines. High hsa_circ_0023404 expression predicted short overall survival in NSCLC. Functionally, knockdown of hsa_circ_0023404 inhibited the proliferation, migration and invasion of NSCLC cells. In the further molecular mechanism study, hsa_circ_0023404 was shown to interact with miR-217/ZEB1 axis to contribute to the growth of NSCLC cells.
hsa_circ_0023404 promotes the proliferation, migration and invasion of NSCLC cells by regulating miR-217/ZEB1 axis, providing a fresh perspective on circRNAs in NSCLC development.
环状RNA（circRNAs）被认为是癌症生物学的关键调节因子。然而，hsa_circ_0023404在非小细胞肺癌（NSCLC）中的功能作用及其调控机制仍几乎未知。
通过定量实时聚合酶链反应评估hsa_circ_0023404、miR-217和锌指E盒结合同源框1（ZEB1）的表达。使用细胞计数试剂盒-8检测、Transwell迁移和侵袭检测确定hsa_circ_0023404在NSCLC进展中的作用。进行荧光素酶报告基因检测以评估hsa_circ_0023404、miR-217和ZEB1在NSCLC细胞中的相互作用。
hsa_circ_0023404在NSCLC组织以及NSCLC细胞系中表达上调。高hsa_circ_0023404表达预示NSCLC患者总生存期较短。在功能上，敲低hsa_circ_0023404可抑制NSCLC细胞的增殖、迁移和侵袭。在进一步的分子机制研究中，hsa_circ_0023404被证明与miR-217/ZEB1轴相互作用，促进NSCLC细胞生长。
hsa_circ_0023404通过调节miR-217/ZEB1轴促进NSCLC细胞的增殖、迁移和侵袭，为circRNAs在NSCLC发生发展中的作用提供了新的视角。
