Suparwitri Sri, Rosyida Niswati Fathmah, Alhasyimi Ananto Ali
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Clin Cosmet Investig Dent. 2019 Aug 9;11:243-249. doi: 10.2147/CCIDE.S212886. eCollection 2019.
The hormone estrogen can prevent osteoclast differentiation and suppress osteoclastic activity, which can prevent tooth relapse movement. Wheat seeds are a natural material containing phytoestrogens with similar characteristics to estrogen.
This study intends to determine the influence of wheat seeds in reducing osteoclastogenesis following orthodontic tooth movement (OTM).
Forty male Sprague Dawley rats were used in this experimental study. The rats were divided into control and treatment groups (n=20). In both groups, the rat maxillary incisors were moved distally using a 0.012 U stainless steel orthodontic appliance with a 2 mm diameter coil and 5 mm wire length. The treatment group received wheat seeds at a dose of 0.6 gram/day. The animals were sacrificed at days 0, 1, 7, and 14 after using the orthodontic appliance (4 subgroups according to observations of days; n=5). RANKL and OPG expressions were determined from the gingival crevicular fluid (GCF) samples using ELISA analysis. Statistical analysis of the data collected was calculated with the independent sample -test (<0.05).
All the experimental procedures were well-tolerated and did not change the animals' weight. On days 7 and 14, the OTM rate of the treatment group was significantly lower than the control group (<0.05). ELISA test results showed that the OPG level was significantly higher on days 1, 7, and 14, while the RANKL level was considerably lower on day 14 in the wheat seeds group compared with the control group (<0.05).
Wheat seeds can promote osteoclastogenesis inhibition and delay OTM.
激素雌激素可阻止破骨细胞分化并抑制破骨细胞活性，从而防止牙齿复发移动。小麦种子是一种天然材料，含有与雌激素特性相似的植物雌激素。
本研究旨在确定小麦种子对正畸牙齿移动（OTM）后破骨细胞生成减少的影响。
本实验研究使用了40只雄性Sprague Dawley大鼠。将大鼠分为对照组和治疗组（n = 20）。在两组中，使用直径2毫米的线圈和5毫米的钢丝长度的0.012 U不锈钢正畸矫治器将大鼠上颌切牙向远中移动。治疗组接受剂量为0.6克/天的小麦种子。在使用正畸矫治器后的第0、1、7和14天处死动物（根据观察天数分为4个亚组；n = 5）。使用ELISA分析从龈沟液（GCF）样本中测定RANKL和OPG表达。对收集的数据进行独立样本检验统计分析（<0.05）。
所有实验程序均耐受性良好，且未改变动物体重。在第7天和第14天，治疗组的OTM率显著低于对照组（<0.05）。ELISA检测结果显示，与对照组相比，小麦种子组在第1、7和14天的OPG水平显著更高，而在第14天的RANKL水平显著更低（<0.05）。
小麦种子可促进破骨细胞生成抑制并延迟OTM。
