Shi Zurong, Wang Qingwei, Li Yasheng, Liang Zhibing, Xu Linghui, Zhou Jianuan, Cui Zining, Zhang Lian-Hui
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2019 Aug 21;10:1950. doi: 10.3389/fmicb.2019.01950. eCollection 2019.
The infections caused by become a severe problem in recent years, but the regulatory mechanisms that govern the bacterial virulence remain to be fragmental. Here we report the investigation of potential involvement of polyamines in regulation of virulence. We showed that null mutation of encoding arginine decarboxylase dramatically decreased the bacterial swimming motility, swarming motility and biofilm formation, and exogenous addition of putrescine effectively rescues the defective phenotypes of . HPLC and mass spectrometry analysis validated that was essential for production of putrescine in . In addition, we demonstrated that EC1 could detect and response to putrescine molecules produced by itself or from host plant through specific transporters. Among the two transporters identified, the one represented by PotF played a dominated role over the other represented by PlaP in modulation of putrescine-dependent biological functions. Furthermore, we provided evidence that putrescine signal is critical for EC1 bacterial invasion and virulence against rice seeds. Our data depict a novel function of putrescine signal in pathogen-host communication and in modulation of the virulence of an important plant bacterial pathogen.
近年来，由[具体细菌名称未给出]引起的感染已成为一个严重问题，但调控细菌毒力的机制仍不完整。在此，我们报告了关于多胺在[具体细菌名称未给出]毒力调控中潜在作用的研究。我们发现，编码精氨酸脱羧酶的[基因名称未给出]的缺失突变显著降低了细菌的泳动性、群集运动性和生物膜形成，而外源添加腐胺可有效挽救[具体细菌名称未给出]的缺陷表型。高效液相色谱和质谱分析证实，[基因名称未给出]对于[具体细菌名称未给出]中腐胺的产生至关重要。此外，我们证明[具体细菌名称未给出]EC1能够通过特定转运蛋白检测并响应自身或宿主植物产生的腐胺分子。在鉴定出的两种转运蛋白中，以PotF为代表的转运蛋白在调节腐胺依赖性生物学功能方面比以PlaP为代表的另一种转运蛋白起主导作用。此外，我们提供的证据表明，腐胺信号对于[具体细菌名称未给出]EC1侵染水稻种子及毒力至关重要。我们的数据揭示了腐胺信号在病原体 - 宿主通讯及调节一种重要植物细菌病原体毒力方面的新功能。
