Huang Wen-Ting, Yang Xia, He Rong-Quan, Ma Jie, Hu Xiao-Hua, Mo Wei-Jia, Chen Gang
Department of Pathology, First Affiliated Hospital of Guangxi Medical University Nanning, Guangxi Zhuang Autonomous Region, P. R. China.
Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University Nanning, Guangxi Zhuang Autonomous Region, P. R. China.
Am J Transl Res. 2019 Aug 15;11(8):4835-4850. eCollection 2019.
Lung adenocarcinoma (LUAD) of non-small cell lung cancer (NSCLC) is a highly prevalent cancer with high mortality. The gene basigin (BSG) is strongly expressed in certain tumors. This study investigated the expression level of BSG in LUAD and its role in the poor prognosis of LUAD.
The mRNA expression of BSG in LUAD was from GEO, Oncomine and TCGA database. Prognostic data were provided by SurvExpress. The expression of BSG protein was detected by immunohistochemistry (IHC). Cell function experiments of viability, proliferation and apoptosis assays were performed in A549. Clustering analysis of BSG co-expressed genes was generated by Gene Ontology (GO) Enrichment, KEGG pathway and protein-protein interaction (PPI) network.
BSG mRNA level was significantly over-expressed in LUAD (pooled SMD = 0.564, 0.448-0.681, P<0.001). The analysis in SurvExpress revealed that high expression of BSG indicated significantly poor prognosis (pooled HR = 1.20, 1.10-1.30, P<0.0001). IHC assay also showed that BSG protein expression was significantly up-regulated in LUAD (P<0.001), and positive BSG expression was notably associated with higher pathology grade (P = 0.041) and lymphatic metastasis (P = 0.014). Moreover, BSG can enhance the viability and proliferation ability (both P<0.001) and weaken cell apoptosis (P<0.001) in A549. The most enriched GO terms in the co-expressed genes of BSG were translation related enrichment. The KEGG pathway showed that these genes were markedly involved in pathways.
Up-regulated BSG in LUAD is related to advanced progression and poor prognosis by influencing cell viability, proliferation and apoptosis. BSG could be a potential biomarker for the targeted therapy of LUAD.
非小细胞肺癌（NSCLC）中的肺腺癌（LUAD）是一种高发性且死亡率高的癌症。基因基底膜联蛋白（BSG）在某些肿瘤中强烈表达。本研究调查了BSG在LUAD中的表达水平及其在LUAD预后不良中的作用。
LUAD中BSG的mRNA表达来自GEO、Oncomine和TCGA数据库。生存数据由SurvExpress提供。通过免疫组织化学（IHC）检测BSG蛋白的表达。在A549细胞中进行了活力、增殖和凋亡检测的细胞功能实验。通过基因本体论（GO）富集、KEGG通路和蛋白质-蛋白质相互作用（PPI）网络对BSG共表达基因进行聚类分析。
LUAD中BSG mRNA水平显著过表达（合并标准化均值差=0.564，0.448 - 0.681，P<0.001）。SurvExpress分析显示，BSG高表达表明预后显著不良（合并风险比=1.20，1.10 - 1.30，P<0.0001）。IHC检测还显示，LUAD中BSG蛋白表达显著上调（P<0.001），且BSG阳性表达与更高的病理分级（P = 0.041）和淋巴转移（P = 0.014）显著相关。此外，BSG可增强A549细胞的活力和增殖能力（均P<0.001）并减弱细胞凋亡（P<0.001）。BSG共表达基因中最富集的GO术语是与翻译相关的富集。KEGG通路显示这些基因明显参与了 通路。
LUAD中上调的BSG通过影响细胞活力、增殖和凋亡与疾病进展和预后不良相关。BSG可能是LUAD靶向治疗的潜在生物标志物。
