Suppr超能文献

长链非编码RNA-DLEU2/微小RNA-186-5p/丙酮酸脱氢酶激酶3轴促进胶质瘤细胞进展。

The lncRNA-DLEU2/miR-186-5p/PDK3 axis promotes the progress of glioma cells.

作者信息

Xie Zuochang, Li Xiaojian, Chen Hua, Zeng Ailiang, Shi Yan, Tang Yong

机构信息

Department of Neurosurgery, The First People's Hospital of Tianmen City Tianmen 431700, Hubei, China.

Department of Neurosurgery, Nanjing First Hospital, Nanjing Medical University Nanjing 210006, Jiangsu, China.

出版信息

Am J Transl Res. 2019 Aug 15;11(8):4922-4934. eCollection 2019.

PMID:31497209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6731438/
Abstract

Long non-coding RNAs (lncRNAs) have great value in research on tumour targeted therapy, including for glioma. In the present study, we investigated the role of the lncRNA deleted in lymphocytic leukaemia 2 (lncRNA-DLEU2) in glioma. First, we found that lncRNA-DLEU2 is highly expressed in glioma tissues and cell lines. Next, experiments in cells showed that lncRNA-DLEU2 knockdown inhibited, whereas lncRNA-DLEU2 overexpression promoted, the clone formation, migration and invasion of glioma cells. A luciferase reporter assay and an RNA immunoprecipitation assay demonstrated that lncRNA-DLEU2 acts as a sponge for miR-186-5p in glioma cells. Further, studies suggested that miR-186-5p inhibits the expression of PDK3, which is an oncogene in glioma. Moreover, with rescue experiments, we demonstrated that lncRNA-DLEU2 regulates the expression of PDK3 and the progression of glioma in a miR-186-5p-dependent manner. Finally, we also showed that lncRNA-DLEU2 promotes glioma growth in a manner that is related to miR-186-5p and PDK3 . In conclusion, our study reported for the first time that lncRNA-DLEU2 promotes glioma progression by targeting the miR-186-5p/PDK3 axis. These findings provide novel strategies for the gene therapy treatment of glioma.

摘要

长链非编码RNA（lncRNAs）在肿瘤靶向治疗研究中具有重要价值，包括对神经胶质瘤的研究。在本研究中，我们调查了淋巴细胞白血病2中缺失的lncRNA（lncRNA-DLEU2）在神经胶质瘤中的作用。首先，我们发现lncRNA-DLEU2在神经胶质瘤组织和细胞系中高表达。接下来，细胞实验表明，敲低lncRNA-DLEU2可抑制神经胶质瘤细胞的克隆形成、迁移和侵袭，而lncRNA-DLEU2过表达则促进这些过程。荧光素酶报告基因检测和RNA免疫沉淀检测表明，lncRNA-DLEU2在神经胶质瘤细胞中充当miR-186-5p的海绵。此外，研究表明miR-186-5p抑制PDK3的表达，而PDK3是神经胶质瘤中的一种癌基因。而且，通过挽救实验，我们证明lncRNA-DLEU2以miR-186-5p依赖的方式调节PDK3的表达和神经胶质瘤的进展。最后，我们还表明lncRNA-DLEU2以与miR-186-5p和PDK3相关的方式促进神经胶质瘤生长。总之，我们的研究首次报道lncRNA-DLEU2通过靶向miR-186-5p/PDK3轴促进神经胶质瘤进展。这些发现为神经胶质瘤的基因治疗提供了新策略。

相似文献

1
The lncRNA-DLEU2/miR-186-5p/PDK3 axis promotes the progress of glioma cells.长链非编码RNA-DLEU2/微小RNA-186-5p/丙酮酸脱氢酶激酶3轴促进胶质瘤细胞进展。
Am J Transl Res. 2019 Aug 15;11(8):4922-4934. eCollection 2019.
2
LncRNA DLEU2 accelerates the tumorigenesis and invasion of non-small cell lung cancer by sponging miR-30a-5p.长链非编码 RNA DLEU2 通过海绵吸附 miR-30a-5p 促进非小细胞肺癌的发生和侵袭。
J Cell Mol Med. 2020 Jan;24(1):441-450. doi: 10.1111/jcmm.14749. Epub 2019 Nov 13.
3
Long non-coding RNA DLEU2 promotes the progression of esophageal cancer through miR-30e-5p/E2F7 axis.长链非编码 RNA DLEU2 通过 miR-30e-5p/E2F7 轴促进食管癌的进展。
Biomed Pharmacother. 2020 Mar;123:109650. doi: 10.1016/j.biopha.2019.109650. Epub 2019 Dec 26.
4
lncRNA DLEU2 Accelerates Oral Cancer Progression via miR-30a-5p/RAP1B Axis to Regulate p38 MAPK Signaling Pathway.长链非编码 RNA DLEU2 通过 miR-30a-5p/RAP1B 轴促进口腔癌进展，从而调节 p38 MAPK 信号通路。
Dis Markers. 2022 Oct 12;2022:9310048. doi: 10.1155/2022/9310048. eCollection 2022.
5
Long noncoding RNA DLEU2 promotes growth and invasion of hepatocellular carcinoma by regulating miR-30a-5p/PTP4A1 axis.长链非编码RNA DLEU2通过调控miR-30a-5p/PTP4A1轴促进肝细胞癌的生长和侵袭。
Pathol Res Pract. 2022 Oct;238:154078. doi: 10.1016/j.prp.2022.154078. Epub 2022 Aug 13.
6
lncRNA DLEU2 modulates cell proliferation and invasion of non-small cell lung cancer by regulating miR-30c-5p/SOX9 axis.长链非编码RNA DLEU2通过调控miR-30c-5p/SOX9轴来调节非小细胞肺癌的细胞增殖和侵袭。
Aging (Albany NY). 2019 Sep 20;11(18):7386-7401. doi: 10.18632/aging.102226.
7
Long noncoding RNA DLEU2 drives the malignant behaviors of thyroid cancer through mediating the miR-205-5p/TNFAIP8 axis.长链非编码RNA DLEU2通过介导miR-205-5p/TNFAIP8轴驱动甲状腺癌的恶性行为。
Endocr Connect. 2021 Apr 26;10(4):471-483. doi: 10.1530/EC-21-0046.
8
LncRNA DLEU2 contributes to Taxol resistance of gastric cancer cells through regulating the miR-30c-5p-LDHA axis.LncRNA DLEU2 通过调节 miR-30c-5p-LDHA 轴促进胃癌细胞对紫杉醇的耐药性。
J Chemother. 2024 Feb;36(1):49-60. doi: 10.1080/1120009X.2023.2203606. Epub 2023 May 9.
9
Knockdown of LncRNA DLEU2 Inhibits Cervical Cancer Progression via Targeting miR-128-3p.下调长链非编码RNA DLEU2通过靶向miR-128-3p抑制宫颈癌进展。
Onco Targets Ther. 2020 Oct 9;13:10173-10184. doi: 10.2147/OTT.S272292. eCollection 2020.
10
lncRNA DLEU2 promotes gastric cancer progression through ETS2 via targeting miR-30a-5p.长链非编码RNA DLEU2通过靶向miR-30a-5p作用于ETS2，从而促进胃癌进展。
Cancer Cell Int. 2021 Jul 14;21(1):376. doi: 10.1186/s12935-021-02074-9.

引用本文的文献

1
Radio-chemotherapy and metformin selectively modulate the heterogeneous landscape of glioma with ribosome biogenesis, long non coding RNA and immune-escape markers as major player.放射化疗和二甲双胍通过以核糖体生物合成、长链非编码RNA和免疫逃逸标志物为主要因素，选择性地调节胶质瘤的异质性格局。
Int J Biol Sci. 2025 May 27;21(8):3527-3554. doi: 10.7150/ijbs.103194. eCollection 2025.
2
Alcohol consumption and esophageal cancer risk: unveiling DLEU2 as a key immune modulator through Mendelian randomization and transcriptomic analysis.饮酒与食管癌风险：通过孟德尔随机化和转录组分析揭示DLEU2作为关键免疫调节因子
Discov Oncol. 2025 May 18;16(1):804. doi: 10.1007/s12672-025-02660-7.
3
Plant Alkaloids as Promising Anticancer Compounds with Blood-Brain Barrier Penetration in the Treatment of Glioblastoma: In Vitro and In Vivo Models.植物生物碱作为有前景的抗癌化合物，具有穿越血脑屏障治疗胶质母细胞瘤的能力：体外和体内模型
Molecules. 2025 Mar 31;30(7):1561. doi: 10.3390/molecules30071561.
4
Deleted in lymphocytic leukemia 2 (DLEU2): a possible biomarker that holds promise for future diagnosis and treatment of cancer.淋巴细胞白血病 2 号缺失（DLEU2）：一种有希望成为癌症未来诊断和治疗的生物标志物。
Clin Transl Oncol. 2023 Oct;25(10):2772-2782. doi: 10.1007/s12094-023-03149-x. Epub 2023 Apr 24.
5
LncRNA LINC01018/miR-942-5p/KNG1 axis regulates the malignant development of glioma in vitro and in vivo.LncRNA LINC01018/miR-942-5p/KNG1 轴调控胶质瘤在体外和体内的恶性发展。
CNS Neurosci Ther. 2023 Feb;29(2):691-711. doi: 10.1111/cns.14053. Epub 2022 Dec 22.
6
PDK1- and PDK2-mediated metabolic reprogramming contributes to the TGFβ1-promoted stem-like properties in head and neck cancer.PDK1和PDK2介导的代谢重编程促进头颈部癌中TGFβ1诱导的干细胞样特性。
Cancer Metab. 2022 Dec 6;10(1):23. doi: 10.1186/s40170-022-00300-0.
7
HEIH Promotes Malignant Progression of Gastric Cancer by Regulating STAT3-Mediated Autophagy and Glycolysis.HEIH 通过调控 STAT3 介导的自噬和糖酵解促进胃癌的恶性进展。
Dis Markers. 2022 Jul 27;2022:2634526. doi: 10.1155/2022/2634526. eCollection 2022.
8
Up-regulation of LINC00665 contributes to the progression of glioma and correlates with its MRI characteristics.LINC00665的上调促进胶质瘤进展并与其MRI特征相关。
Am J Transl Res. 2022 May 15;14(5):2988-3002. eCollection 2022.
9
LINC00022 acts as an oncogene in colorectal cancer progression via sponging miR-375-3p to regulate FOXF1 expression.LINC00022 通过海绵吸附 miR-375-3p 调控 FOXF1 表达，在结直肠癌进展中发挥癌基因作用。
BMC Cancer. 2022 Apr 26;22(1):453. doi: 10.1186/s12885-022-09566-5.
10
LINC01087 inhibits glioma cell proliferation and migration, and increases cell apoptosis via miR-384/Bcl-2 axis.LINC01087 通过 miR-384/Bcl-2 轴抑制神经胶质瘤细胞增殖和迁移，并增加细胞凋亡。
Aging (Albany NY). 2021 Aug 30;13(16):20808-20819. doi: 10.18632/aging.203478.

本文引用的文献

1
Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT.竞争性内源性 RNA 是 EMT 调控回路的固有组成部分，并调节 EMT。
Nat Commun. 2019 Apr 9;10(1):1637. doi: 10.1038/s41467-019-09649-1.
2
Investigation of cytotoxic, genotoxic, and apoptotic effects of curcumin on glioma cells.姜黄素对胶质瘤细胞的细胞毒性、遗传毒性和凋亡作用的研究。
Cell Mol Biol (Noisy-le-grand). 2019 Mar 31;65(3):101-108.
3
Serum Levels of APRIL Increase in Patients with Glioma, Meningioma and Schwannoma.胶质瘤、脑膜瘤和神经鞘瘤患者血清增殖诱导配体（APRIL）水平升高。
Asian Pac J Cancer Prev. 2019 Mar 26;20(3):751-756. doi: 10.31557/APJCP.2019.20.3.751.
4
LncRNA SNHG16 promotes proliferation, migration and invasion of osteosarcoma cells by targeting miR-1301/BCL9 axis.长链非编码 RNA SNHG16 通过靶向 miR-1301/BCL9 轴促进骨肉瘤细胞的增殖、迁移和侵袭。
Biomed Pharmacother. 2019 Jun;114:108798. doi: 10.1016/j.biopha.2019.108798. Epub 2019 Mar 22.
5
miR-128-3p inhibits glioma cell proliferation and differentiation by targeting NPTX1 through IRS-1/PI3K/AKT signaling pathway.微小RNA-128-3p通过IRS-1/PI3K/AKT信号通路靶向NPTX1抑制胶质瘤细胞的增殖和分化。
Exp Ther Med. 2019 Apr;17(4):2921-2930. doi: 10.3892/etm.2019.7284. Epub 2019 Feb 18.
6
Long Noncoding RNA LINC00202 Promotes Tumor Progression by Sponging miR-3619-5p in Retinoblastoma.长链非编码RNA LINC00202通过海绵化视网膜母细胞瘤中的miR-3619-5p促进肿瘤进展。
Cell Struct Funct. 2019;44(1):51-60. doi: 10.1247/csf.18033.
7
The Long Noncoding RNA NEAT1 Targets miR-34a-5p and Drives Nasopharyngeal Carcinoma Progression via Wnt/β-Catenin Signaling.长链非编码RNA NEAT1靶向miR-34a-5p并通过Wnt/β-连环蛋白信号通路驱动鼻咽癌进展。
Yonsei Med J. 2019 Apr;60(4):336-345. doi: 10.3349/ymj.2019.60.4.336.
8
Glioma Tumor Grade Identification Using Artificial Intelligent Techniques.利用人工智能技术进行胶质瘤肿瘤分级识别。
J Med Syst. 2019 Mar 21;43(5):113. doi: 10.1007/s10916-019-1228-2.
9
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients.在原发性胶质母细胞瘤患者中，miR-21、miR-34a、miR-125b、miR-181d和miR-648的水平与MGMT和TP53的表达呈负相关。
Arch Med Sci. 2019 Mar;15(2):504-512. doi: 10.5114/aoms.2017.69374. Epub 2017 Jul 31.
10
TLR2 promotes development and progression of human glioma via enhancing autophagy.TLR2 通过增强自噬促进人神经胶质瘤的发生发展。
Gene. 2019 Jun 5;700:52-59. doi: 10.1016/j.gene.2019.02.084. Epub 2019 Mar 19.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验