Zhang Li Hua, Li Long Hai, Zhang Peng Fei, Cai Yan Fei, Hua Dong
Affiliated Hospital of Jiangnan University, Jiangnan University, Wuxi, China.
School of Pharmaceutical Science, Jiangnan University, Wuxi, China.
Front Oncol. 2019 Aug 21;9:776. doi: 10.3389/fonc.2019.00776. eCollection 2019.
Colorectal cancer (CRC) is one of the most prevalent digestive tumors in China. Recent studies indicate that long intergenic non-coding RNAs (lincRNAs) play a crucial role in predicting survival for CRC patients. However, the novel lincRNA, LINC00957, is largely unclear in CRC. The purpose of the current study was to determine LINC00957 expression, assess its the clinical significance and explore the potential mechanism in CRC. The qRT-PCR was used to quantify the expression levels of LINC00957 in tissues and cell lines. Our research revealed that LINC00957 was significantly higher expression in CRC. In addition, the LINC00957 expression was associated with TNM stage and chemotherapy outcome, but age, gender, tumor size, histological grade, primary tumor location. CRC patients with high LINC00957 expression level showed poor overall survival ( = 0.002). Multivariate survival analysis indicated that LINC00957 was a prognostic factor for CRC patients ( = 0.010). Mechanically, inhibition of LINC00957 expression reversed 5-FU resistance by down-regulating P-gP. In summary, our study indicated that this novel lncRNA expression signature might be a useful biomarker of the prognosis and therapeutic target for CRC patients.
结直肠癌（CRC）是中国最常见的消化道肿瘤之一。最近的研究表明，长链基因间非编码RNA（lincRNAs）在预测CRC患者的生存方面起着关键作用。然而，新型lincRNA LINC00957在CRC中的情况在很大程度上尚不清楚。本研究的目的是确定LINC00957的表达，评估其临床意义并探索其在CRC中的潜在机制。采用qRT-PCR定量检测组织和细胞系中LINC00957的表达水平。我们的研究表明，LINC00957在CRC中的表达显著更高。此外，LINC00957的表达与TNM分期和化疗结果相关，但与年龄、性别、肿瘤大小、组织学分级、原发肿瘤位置无关。LINC00957表达水平高的CRC患者总生存期较差（P = 0.002）。多因素生存分析表明，LINC00957是CRC患者的一个预后因素（P = 0.010）。机制上，抑制LINC00957的表达通过下调P-gP逆转了5-FU耐药性。总之，我们的研究表明，这种新型lncRNA表达特征可能是CRC患者预后的有用生物标志物和治疗靶点。
