Chaleshi Vahid, Irani Shiva, Alebouyeh Masoud, Mirfakhraie Reza, Aghdaei Hamid Asadzadeh
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717413, Iran.
Oncol Lett. 2020 Jun;19(6):3937-3949. doi: 10.3892/ol.2020.11518. Epub 2020 Apr 7.
Colorectal cancer (CRC) is a common intestinal cancer with a high mortality rate. Early detection of this type of cancer is fundamental to the prevention of the disease, which results in improved survival rates. In the human colon tissue, transition from normal epithelium to adenoma is considered to be caused by unknown molecular incidents occurring over 5-10 years. The detection of CRC has proved problematic when in the early stages of disease. In addition, identifying suitable biomarkers for the detection of CRC progress in patients remains one of the most significant challenges. Long non-coding RNAs have been demonstrated to contribute to the promotion of CRC. The aim of the present study was to investigate the clinical and biological significance of long intergenic non-coding (linc)RNA-p21, lincRNA-regulator of reprogramming (ROR) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in the colon tumor and polyp tissue, and the association that these have with the expression of p53 at the mRNA level. Neoplastic and paired adjacent normal tissue samples were obtained from 72 patients (46 polyps and 26 tumors). Reverse transcription-quantitative PCR was performed to determine the relative fold changes in the expression of lincRNA-p21, lincRNA-RoR, MALAT1 and p53 in the samples. A significant association was observed between the levels of MALAT1 and p53 in neoplasm tissues (R=0.073; P<0.05). The relative expression of the MALAT1 gene revealed a statistically significant difference between the different polyp types and number of polyps (P=0.0028 and 0.022, respectively). Adjuvant therapy in patients with tumors revealed an association between the levels of lincRNA-ROR and lincRNA-p21 expression (P=0.015 and 0.038, respectively). MALAT1 may be selected as an early detection biomarker for CRC. Furthermore, lincRNA-ROR and lincRNA-p21 may serve as prognostic and therapeutic biomarkers in patients with CRC.
结直肠癌（CRC）是一种常见的肠道癌症，死亡率很高。早期发现这种类型的癌症是预防该疾病的基础，这会提高生存率。在人类结肠组织中，从正常上皮向腺瘤的转变被认为是由5至10年内发生的未知分子事件引起的。在疾病早期，CRC的检测已被证明存在问题。此外，为患者检测CRC进展确定合适的生物标志物仍然是最重大的挑战之一。长链非编码RNA已被证明有助于促进CRC。本研究的目的是调查长链基因间非编码（linc）RNA-p21、重编程调节因子（ROR）的lincRNA和转移相关肺腺癌转录本1（MALAT1）在结肠肿瘤和息肉组织中的临床和生物学意义，以及它们与mRNA水平的p53表达之间的关联。从72例患者（46例息肉和26例肿瘤）中获取肿瘤及配对的相邻正常组织样本。进行逆转录定量PCR以确定样本中lincRNA-p21、lincRNA-RoR、MALAT1和p53表达的相对倍数变化。在肿瘤组织中观察到MALAT1和p53水平之间存在显著关联（R = 0.073；P < 0.05）。MALAT1基因的相对表达在不同息肉类型和息肉数量之间显示出统计学上的显著差异（分别为P = 0.0028和0.022）。肿瘤患者的辅助治疗显示lincRNA-ROR和lincRNA-p21表达水平之间存在关联（分别为P = 0.015和0.038）。MALAT1可被选作CRC的早期检测生物标志物。此外，lincRNA-ROR和lincRNA-p21可作为CRC患者的预后和治疗生物标志物。
