Abdolmaleki Fahimeh, Zamani Zahra, Talebi Somayeh
Department of Biochemistry, Tehran East Branch, Payam Noor University, Tehran, Iran.
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Iran J Public Health. 2019 Jul;48(7):1326-1334.
Egg yolk is a rich and accessible source of yolk immunoglobulin (Y immunoglobulin). Presently, polyclonal antibodies from mammalian sources are used for diagnosis. Antibody production from egg yolk gives a higher yield and turnover than that from lab animals, and invasive methods such as phlebotomy and causing stress to the animals are not required. Due to the issues regarding mammalian antibodies, we aimed to evaluate the human anti-IgG polyclonal antibody production conjugated with peroxidase in egg yolk.
Population of laying hens reared in Agriculture/Isfahan University of Technology were used in 2017. After immunizing hen against pure human IgG, specific IgY (yolk immunoglobulin) was purified from the yolk by sedimentation with polyethylene glycol (PEG6000). To assess the molecular weight and activity of the product, SDS-PAGE and ELISA-test were used, respectively.
The complete molecular weight of IgY was 180 kDa and the molecular weight of its light and heavy chains were 27 and 67 kDa, respectively.
Antihuman IgG IgY had a purity above 90%. The product of this study can be used to measure IgG class antibodies in order to diagnose different diseases.
蛋黄是卵黄免疫球蛋白（Y免疫球蛋白）丰富且易于获取的来源。目前，用于诊断的多克隆抗体来源于哺乳动物。与从实验动物获取抗体相比，从蛋黄中生产抗体的产量更高且周转更快，并且不需要诸如静脉穿刺和给动物造成应激等侵入性方法。鉴于哺乳动物抗体存在的问题，我们旨在评估在蛋黄中生产与过氧化物酶偶联的人抗IgG多克隆抗体。
2017年使用了在农业/伊斯法罕理工大学饲养的蛋鸡群体。用纯人IgG免疫母鸡后，通过用聚乙二醇（PEG6000）沉淀从蛋黄中纯化特异性IgY（卵黄免疫球蛋白）。分别使用SDS-PAGE和ELISA试验评估产物的分子量和活性。
IgY的完整分子量为180 kDa，其轻链和重链的分子量分别为27 kDa和67 kDa。
抗人IgG IgY的纯度高于90%。本研究的产物可用于检测IgG类抗体以诊断不同疾病。
