Li X, Nakano T, Sunwoo H H, Paek B H, Chae H S, Sim J S
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Poult Sci. 1998 Feb;77(2):266-70. doi: 10.1093/ps/77.2.266.
Twenty 35-wk-old chickens, including 10 Single Comb White Leghorn (SCWL) and 10 Rhode Island Red (RIR) hens, were used to examine the effects of egg and yolk weights on egg yolk antibody (IgY) production in the two strains of chickens immunized with BSA. The SCWL chickens had a greater (P < 0.01) percentage hen-day production and greater egg and yolk weights than did the RIR chickens. However, the anti-BSA antibody activities determined by ELISA in the serum and the egg yolk were similar (P > 0.05) between the SCWL and RIR chickens. Similarities between the two strains of hens were also observed in protein and total IgY contents (expressed as the percentage of wet weight of yolk) and the percentage of BSA-specific antibody in the total IgY. It was concluded that both the SCWL and RIR chickens immunized with BSA can produce egg yolk IgY containing similar proportions of BSA-specific antibodies. Therefore, the egg yolk weight and the percentage hen-day production, both of which are greater in the SCWL hens, are considered to be important factors for the efficient production of IgY.
选用20只35周龄的母鸡，其中包括10只单冠白来航鸡（SCWL）和10只罗德岛红鸡（RIR），用于研究鸡蛋和蛋黄重量对用牛血清白蛋白（BSA）免疫的这两种品系鸡的蛋黄抗体（IgY）产生的影响。与罗德岛红鸡相比，单冠白来航鸡的日产蛋率更高（P < 0.01），鸡蛋和蛋黄重量也更大。然而，通过酶联免疫吸附测定法（ELISA）测定，单冠白来航鸡和罗德岛红鸡血清及蛋黄中的抗牛血清白蛋白抗体活性相似（P > 0.05）。在蛋白质和总IgY含量（以蛋黄湿重的百分比表示）以及总IgY中牛血清白蛋白特异性抗体的百分比方面，也观察到这两种品系母鸡之间存在相似性。得出的结论是，用牛血清白蛋白免疫的单冠白来航鸡和罗德岛红鸡都能产生含有相似比例牛血清白蛋白特异性抗体的蛋黄IgY。因此，单冠白来航鸡中更高的蛋黄重量和日产蛋率被认为是高效生产IgY的重要因素。
