Xu Lili, Fan Jianhua, Wang Quanxi
Department of Biology, College of Life Sciences, Shanghai Normal University, Shanghai, China.
State Key Laboratory of South China Sea Marine Resource Utilization, Hainan University, Haikou, China.
Front Bioeng Biotechnol. 2019 Aug 21;7:201. doi: 10.3389/fbioe.2019.00201. eCollection 2019.
This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in . In this paper, critical genes () involved in H metabolism were identified and analyzed for their function in H accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H metabolism. This review provides fundamental theory and an experimental basis for H production, and further research effort is needed in this field.
本文总结了在生物制氢背景下，关于组学方法（包括基因组学、转录组学、蛋白质组学和代谢组学）的当前知识。在本文中，鉴定并分析了参与氢代谢的关键基因及其在氢积累中的功能。此外，比较了基因芯片和RNA测序的优势，以及它们在氢生产转录组分析中的应用。此外，作为一种有用的工具，蛋白质组学分析可以鉴定参与氢代谢的不同蛋白质。本综述为氢生产提供了基础理论和实验依据，该领域还需要进一步的研究努力。
