School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran; Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edif. Severo Ochoa, 14071 Córdoba, Spain.
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Campus de Rabanales, Edif. Severo Ochoa, 14071 Córdoba, Spain.
Bioresour Technol. 2019 Oct;289:121648. doi: 10.1016/j.biortech.2019.121648. Epub 2019 Jun 13.
This study is a proof of concept for the synergetic biohydrogen production in alga-bacteria co-cultures. Algal hydrogen photoproduction was obtained in sugar-containing media only when the green alga Chlamydomonas reinhardtii was co-cultured with Pseudomonas putida (40.8 ml H·L), Escherichia coli (35.1 ml H·L) and Rhizobium etli (16.1 ml H·L). Hydrogen photo-production in these co-cultures was not only linked to the induction of hypoxia, but to the ability of the bacteria to produce acetic acid from sugars. Synergetic hydrogen production was achieved by integrating the photobiological and fermentative production in Chlamydomonas and Escherichia coli co-cultures supplemented with glucose, which resulted in 60% more H production than the sum of the respective monocultures. This cooperation relied on the ability of the alga to consume the excreted bacterial acetic acid, which benefited both bacterial and algal hydrogen production. This knowledge may open new possibilities for the biohydrogen production from industrial wastes.
本研究证明了藻类-细菌共培养物中的协同生物制氢作用。只有当绿藻莱茵衣藻与假单胞菌（40.8ml H·L）、大肠杆菌（35.1ml H·L）和根瘤菌（16.1ml H·L）共培养时，在含有糖的培养基中才能获得藻类的光合氢气生产。这些共培养物中的氢气光生产不仅与缺氧的诱导有关，还与细菌将糖转化为乙酸的能力有关。通过整合补充葡萄糖的莱茵衣藻和大肠杆菌共培养物中的光合生物和发酵生产，实现了协同制氢，其产氢量比各自的单培养物总和增加了 60%。这种合作依赖于藻类消耗细菌分泌的乙酸的能力，这对细菌和藻类的氢气生产都有利。这一知识可能为利用工业废物生产生物氢气开辟新的可能性。
