基于微流控液滴一步法制备固定化酶的可重复使用聚合微胶囊
One-Step Fabrication of Enzyme-Immobilized Reusable Polymerized Microcapsules from Microfluidic Droplets.
作者信息
Varshney Rohit, Sharma Shubhra, Prakash Bhanu, Laha Joydev K, Patra Debabrata
机构信息
Habitat Centre, Institute of Nano Science and Technology, Phase-10, Sector-64, Mohali, Punjab 160062, India.
Department of Pharmaceutical Technology (Process Chemistry), National Institute of Pharmaceutical Education and Research, S. A. S. Nagar, Mohali, Punjab 160062, India.
出版信息
ACS Omega. 2019 Aug 12;4(9):13790-13794. doi: 10.1021/acsomega.9b01321. eCollection 2019 Aug 27.
Enzyme immobilization is an essential prerequisite for biocatalysis. In this context, emulsion provides an excellent template for assembling enzymes at the oil-water interface. A microfluidic approach has been adopted to produce oil-in-water-type emulsions stabilized by gold nanoparticle-catalase conjugates. In situ ring-opening polymerization of the oil phase produces solid core enzyme-immobilized microcapsules (MCs). These resultant MCs exhibited a value of 42 mM and shows 1.1-fold higher activity compared to free enzymes. Finally, the robust MCs showed excellent recyclability, which can meet the demand of industrial biotechnological applications.
酶固定化是生物催化的一个重要前提条件。在这种情况下，乳液为在油水界面组装酶提供了一个优良的模板。已采用微流控方法来制备由金纳米颗粒 - 过氧化氢酶共轭物稳定的水包油型乳液。油相的原位开环聚合产生了固定有酶的实心微胶囊（MCs）。这些所得的微胶囊表现出42 mM的值，并且与游离酶相比活性高1.1倍。最后，坚固的微胶囊显示出优异的可回收性，能够满足工业生物技术应用的需求。
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