Domingo J L, Llobet J M, Colomina M T, Corbella J
Laboratory of Toxicology and Biochemistry, School of Medicine, University of Barcelona, Spain.
Vet Hum Toxicol. 1988 Dec;30(6):524-7.
Effects of ip treatment with diethylenetriaminepentaacetic acid (DTPA), and cyclohexanediaminetetraacetic acid (CDTA) on the zinc (Zn) excretion and Zn levels in selected mouse organs and tissues were assessed after mice were offered deionized water containing zinc acetate dihydrate (108 mg/kg/day) as the sole drinking fluid for 4 weeks. Following this period, the Zn-containing water was replaced by tap water and therapy with DTPA or CDTA was initiated. The animals received 6 injections of chelators or 0.9% saline (control group) on alternate days for 2 weeks of treatment. The dose of chelating agents was approximately equal to 1/4 of their respective ip LD50 values. Mice were housed in metabolic cages, and urine and feces were collected 24 hr after the first, fourth and sixth administration of the chelators. Six animals in each group were sacrificed at the same days. Although feces was the predominant route of elimination for Zn, only DTPA significantly increased the fecal excretion of Zn after the first administration of chelator. Treatment with DTPA or CDTA resulted in a significant decrease in the concentration of Zn in brain, spleen, and heart after the first injection. DTPA was consistently the most effective in increasing the urinary and fecal excretion of Zn and reducing the concentration of the metal found in various tissues. CDTA would be considered as a possible alternative.
在用含有二水合醋酸锌（108毫克/千克/天）的去离子水作为唯一饮用水源给小鼠饮用4周后，评估了腹腔注射二乙烯三胺五乙酸（DTPA）和环己二胺四乙酸（CDTA）对锌（Zn）排泄以及所选小鼠器官和组织中锌水平的影响。在此期间过后，将含锌水换成自来水，并开始用DTPA或CDTA进行治疗。动物每隔一天接受6次螯合剂或0.9%生理盐水注射（对照组），持续治疗2周。螯合剂的剂量约等于其各自腹腔注射半数致死量值的四分之一。小鼠饲养在代谢笼中，在首次、第四次和第六次注射螯合剂后24小时收集尿液和粪便。每组在同一天处死6只动物。尽管粪便一直是锌排泄的主要途径，但仅在首次注射螯合剂后，DTPA显著增加了锌的粪便排泄量。首次注射后，用DTPA或CDTA治疗导致大脑、脾脏和心脏中锌的浓度显著降低。DTPA在增加锌的尿液和粪便排泄量以及降低各种组织中金属浓度方面一直是最有效的。CDTA可被视为一种可能的替代物。
