Wieczorek H, Oberdörster G
Pol J Occup Med. 1989;2(4):389-96.
The effect of 1, 2-cyclohexylene-aminetetraacetic acid (CDTA) and diethylenetriaminepentaacetic acid (DTPA) on Min distribution and excretion in rats was examined after 1 hr exposure to 54MnCl2 (0.3 micrograms Mn/m3). Both chelating agents were inhaled independently, by nose only, for 30 min for the four following days, starting immediately after cessation of Mn exposure. During the experiment, the mean concentrations of CDTA and DTPA aerosols were 508 mg/cm3 and 517 mg/cm3, respectively. The activity of 54Mn was determined in lung, liver, kidney and brain 24 hrs after the last treatment, and in urine and faeces collected for 24 hrs on days 1-4 after Mn exposure. The CDTA inhalation appeared to be more effective than DTPA in mobilizing the inhaled manganese: about a twofold decrease of 54Mn was observed in all excised organs: lung, liver, kidney and brain, as compared to the controls. The DTPA inhalation resulted in about a twofold decrease of 54Mn in the lung, but was not effective in removing the metal from the liver, kidney and brain. As it can be seen from the comparison of these results with our previous data (6), the CDTA inhalations were more effective than its i.p. injections mobilizing manganese additionally also from the lung; but generally less effective in the case of DTPA, which decreased 54Mn levels in the lung only. On the first day after 54Mn exposure, manganese levels in the urine of rats inhaling CDTA and DTPA were respectively more than 200 and 30 times higher than in the control group. Excretion of Mn in faeces was not affected significantly in this experiment. Our data show that the effectiveness of removing inhaled Mn depends not only on the chelating agent, the time of its administration (see Part I) but also on the method of its administration.
在大鼠暴露于54MnCl2（0.3微克锰/立方米）1小时后，研究了1,2 - 环己二胺四乙酸（CDTA）和二亚乙基三胺五乙酸（DTPA）对大鼠体内锰分布和排泄的影响。在停止锰暴露后立即开始，随后四天每天仅经鼻独立吸入这两种螯合剂30分钟。实验期间，CDTA和DTPA气雾剂的平均浓度分别为508毫克/立方厘米和517毫克/立方厘米。在最后一次处理24小时后，测定肺、肝、肾和脑中54Mn的活性，并在锰暴露后第1 - 4天收集24小时的尿液和粪便中测定54Mn的活性。吸入CDTA在调动吸入的锰方面似乎比DTPA更有效：与对照组相比，在所有切除的器官（肺、肝、肾和脑）中观察到54Mn含量约降低了两倍。吸入DTPA使肺中54Mn含量降低了约两倍，但在从肝、肾和脑中去除金属方面无效。从这些结果与我们先前数据（6）的比较可以看出，吸入CDTA比腹腔注射更有效，还能从肺中调动额外的锰；但对于DTPA而言通常效果较差，DTPA仅降低了肺中54Mn的水平。在54Mn暴露后的第一天，吸入CDTA和DTPA的大鼠尿液中的锰水平分别比对照组高200多倍和30倍以上。在本实验中，粪便中锰的排泄没有受到显著影响。我们的数据表明，去除吸入的锰的有效性不仅取决于螯合剂、给药时间（见第一部分），还取决于给药方法。
