Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Programa de Pós-graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Laboratório de Biogenômica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Programa de Pós-graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Food Chem Toxicol. 2019 Dec;134:110809. doi: 10.1016/j.fct.2019.110809. Epub 2019 Sep 6.
The role of caffeinated beverages on efficiency of acute inflammatory responses is not yet fully understood. This study analyzed the effect of five hot water extracts, coffee (CO), black/green tea (BT/GT), yerba mate (YM), and guarana (GU) on inflammatory modulation of non-activated human peripheral blood mononuclear cells (PBMCs), yeast-activated human neutrophils, and granulocytic coelomocytes from Eisenia fetida earthworm. Based on preliminary tests, a concentration of 10 μg/mL was chosen for subsequent assays, as at this concentration, the extracts exhibited antioxidant, genoprotective, and non-cytotoxic properties. Immunoassays using 24-h PBMC supernatant showed that all extracts decreased levels of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ), and increased levels of the anti-inflammatory cytokine, IL-10. Further, these extracts induced overexpression of cytokine genes in 24-h cultures. These results suggest that an increase in the levels of mRNAs and/or inactive cytokines in the cytoplasm improves the "immune cytokine response. Analysis of the yeast encapsulation processes, and production of human neutrophils and coelomocyte extracellular DNA traps suggests that extracts also improve the immune response in humans and earthworms. However, for E. fetida, the intensity of these results varied from extract. Overall, our results suggest that caffeinated beverages may improve an organism's efficiency against acute inflammatory processes.
咖啡因饮料对急性炎症反应效率的影响尚不完全清楚。本研究分析了五种热水提取物，即咖啡（CO）、黑茶/绿茶（BT/GT）、马黛茶（YM）和瓜拉那（GU）对非激活人外周血单核细胞（PBMC）、酵母激活的人嗜中性粒细胞和赤子爱胜蚓（Eisenia fetida）的粒细胞体腔细胞的炎症调节作用。基于初步测试，选择了 10μg/mL 的浓度用于后续实验，因为在该浓度下，提取物表现出抗氧化、基因保护和非细胞毒性特性。使用 24 小时 PBMC 上清液进行免疫分析表明，所有提取物均降低了促炎细胞因子（IL-1β、IL-6、TNF-α 和 IFN-γ）的水平，增加了抗炎细胞因子 IL-10 的水平。此外，这些提取物在 24 小时培养物中诱导细胞因子基因的过表达。这些结果表明，细胞质中细胞因子 mRNA 和/或无活性细胞因子水平的增加改善了“免疫细胞因子反应”。对酵母包封过程的分析，以及人嗜中性粒细胞和体腔细胞细胞外 DNA 陷阱的产生表明，提取物还改善了人类和赤子爱胜蚓的免疫反应。然而，对于赤子爱胜蚓，这些结果的强度因提取物而异。总的来说，我们的结果表明，含咖啡因的饮料可能提高生物体对急性炎症过程的效率。
