微小 RNA-299-3p/叉头框蛋白 P4 轴调节口腔鳞状细胞癌的增殖和迁移。
MicroRNA-299-3p/FOXP4 Axis Regulates the Proliferation and Migration of Oral Squamous Cell Carcinoma.
机构信息
Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People's Republic of China.
Department of Oral Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People's Republic of China.
出版信息
Technol Cancer Res Treat. 2019 Jan 1;18:1533033819874803. doi: 10.1177/1533033819874803.
MicroRNAs are noncoding RNAs of 21 to 23 nucleotides in length that play important roles in almost all biological pathways. The roles of microRNA-299-3p in the development and progression of oral squamous cell carcinoma remain unclear. Expression level of microRNA-299-3p in oral squamous cell carcinoma cell lines was analyzed. Then, the effects of microRNA-299-3p on oral squamous cell carcinoma cell proliferation and migration were investigated. Moreover, bioinformation algorithm and Western blot were conducted to explore whether forkhead box P4 was a direct target of miR-299-3p. We showed that microRNA-299-3p expression was significantly reduced in oral squamous cell carcinoma cell lines. Next, overexpression of microRNA-299-3p was found to inhibit oral squamous cell carcinoma cell proliferation and migration but promote apoptosis. In addition, forkhead box P4 was identified as a functional target of microRNA-299-3p. Our results provide a new perspective for the mechanisms underlying the progression of oral squamous cell carcinoma and a novel target for the treatment of oral squamous cell carcinoma.
微小 RNA 是长度为 21 到 23 个核苷酸的非编码 RNA，在几乎所有的生物途径中都发挥着重要作用。微小 RNA-299-3p 在口腔鳞状细胞癌的发展和进展中的作用尚不清楚。分析了口腔鳞状细胞癌细胞系中微小 RNA-299-3p 的表达水平。然后，研究了微小 RNA-299-3p 对口腔鳞状细胞癌细胞增殖和迁移的影响。此外，还进行了生物信息学算法和 Western blot 实验，以探讨叉头框 P4 是否是 miR-299-3p 的直接靶标。结果表明，微小 RNA-299-3p 在口腔鳞状细胞癌细胞系中的表达明显降低。接下来，发现微小 RNA-299-3p 的过表达可抑制口腔鳞状细胞癌细胞的增殖和迁移，但可促进凋亡。此外，还鉴定出叉头框 P4 是微小 RNA-299-3p 的功能靶标。我们的研究结果为口腔鳞状细胞癌进展的机制提供了新的视角，并为口腔鳞状细胞癌的治疗提供了新的靶点。
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