Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Andrologia. 2019 Nov;51(10):e13406. doi: 10.1111/and.13406. Epub 2019 Sep 9.
Although many studies reported the detrimental effects of type 1 and 2 diabetes mellitus (T1DM and T2DM) on testis, reproductive parameter changes in DM seminal vesicles have never been documented. This study aimed to examine the morphology, biochemical levels and tyrosine phosphorylation in seminal vesicles of T1DM and T2DM mice. Fifty-six male C57BL/6 mice were divided into four groups (n = 14/each): T1DM control, T1DM, T2DM control and T2DM. T1DM mice were daily injected of streptozotocin (STZ; 40 mg/kg BW) for 5 days. T2DM mice received high-fat diet for 14 days prior to STZ injection at a single dose (85 mg/kg BW). At the end of experiments (days 36 and 72), magnesium (MG) and fructosamine (FRA) levels, and phosphorylated protein expression in seminal vesicle were examined. The results showed that seminal and prostate weights and MG and FRA levels of T1DM animals were significantly increased as compared to T2DM mice. Some seminal histopathologies and decreased epithelial height were observed in both DM groups. Significantly, a 72-kDa phosphorylated protein expression was increased in DM seminal vesicle. We concluded that changes of biochemical components and phosphorylated proteins in seminal vesicle of T1DM and T2DM mice may be associated with low-quality seminal plasma.
尽管许多研究报告了 1 型和 2 型糖尿病（T1DM 和 T2DM）对睾丸的有害影响，但 DM 精囊的生殖参数变化从未有过记录。本研究旨在检查 T1DM 和 T2DM 小鼠精囊的形态、生化水平和酪氨酸磷酸化。56 只雄性 C57BL/6 小鼠分为四组（每组 n = 14）：T1DM 对照组、T1DM、T2DM 对照组和 T2DM。T1DM 小鼠每天注射链脲佐菌素（STZ；40mg/kg BW）连续 5 天。T2DM 小鼠接受高脂肪饮食 14 天，然后单次注射 STZ（85mg/kg BW）。在实验结束时（第 36 天和第 72 天），检查精囊的镁（MG）和果糖胺（FRA）水平以及磷酸化蛋白的表达。结果显示，与 T2DM 小鼠相比，T1DM 动物的精液和前列腺重量以及 MG 和 FRA 水平显著增加。在两个 DM 组中都观察到一些精液组织病理学变化和上皮高度降低。值得注意的是，DM 精囊中的 72 kDa 磷酸化蛋白表达增加。我们得出结论，T1DM 和 T2DM 小鼠精囊的生化成分和磷酸化蛋白的变化可能与低质量精液有关。
