Sun Lei, Zhuang Lv-Ping, Wu Wei-Feng
Sports Teaching and Research Department, Fujian Medical University, Fuzhou, Fujian Province, China.
Department of Neurology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China.
An Acad Bras Cienc. 2019;91(3):e20190068. doi: 10.1590/0001-3765201920190068. Epub 2019 Sep 9.
To investigate the mechanism of different exercise patterns on neurological function after focal cerebral ischaemia in rats. Rats with focal cerebral cerebral ischaemia were randomly divided into an aerobic exercise group, an exhaustive exercise group and a control group, with 8 rats in each group. A score for nerve function in each group was calculated, and the ultrastructure of nerve cells was observed. Levels of NO and NOS in the brain motor area of the rats were measured in each group. The aerobic exercise group had lower nerve function scores than the exhaustive exercise group and higher scores than the control group (P<0.05). Under transmission electron microscopy, irregular shapes and organs were observed in nerve cells in the control group, while regular cell shapes and organs were observed in the aerobic exercise group. The aerobic exercise group and exhaustive exercise group had higher measures of NO content, NOS activity and eNOS, nNOS and iNOS gene expression than the control group, but eNOS expression in the aerobic exercise group and iNOS expression in the exhaustive exercise group were clearly higher according to RT-PCR (P<0.05). Aerobic exercise can promote the expression of NOS, mainly in eNOS, which can promote nerve repair.
为探讨不同运动模式对大鼠局灶性脑缺血后神经功能的影响机制。将局灶性脑缺血大鼠随机分为有氧运动组、力竭运动组和对照组，每组8只。计算每组神经功能评分，并观察神经细胞超微结构。检测每组大鼠脑运动区一氧化氮（NO）和一氧化氮合酶（NOS）水平。有氧运动组神经功能评分低于力竭运动组，高于对照组（P<0.05）。透射电镜下，对照组神经细胞形态和细胞器不规则，而有氧运动组细胞形态和细胞器规则。有氧运动组和力竭运动组的NO含量、NOS活性及内皮型NOS（eNOS）、神经元型NOS（nNOS）和诱导型NOS（iNOS）基因表达均高于对照组，但逆转录-聚合酶链反应（RT-PCR）结果显示，有氧运动组eNOS表达及力竭运动组iNOS表达明显更高（P<0.05）。有氧运动可促进NOS表达，主要是eNOS表达，从而促进神经修复。
