Shui Ling, Yi Ru-Na, Wu Yong-Jie, Bai Shu-Mei, Si Qin, Bo A-Gula, Wuyun Ge-Rile, Si Leng-Ge, Chen Ying-Song, Lu Jun
College of Traditional Mongolia Medicine, Inner Mongolia Medical University, Hohhot, China.
School of Acupuncture-Moxibustion and Tui Na, Beijing University of Chinese Medicine, Beijing, China.
Front Integr Neurosci. 2020 Jan 31;13:78. doi: 10.3389/fnint.2019.00078. eCollection 2019.
The inducible nitric oxide synthase/nitric oxide (iNOS/NO) signaling pathway and inflammatory cytokines play important roles in the pathogenesis of exercise-induced fatigue. Studies have found that Mongolian warm acupuncture (WA) could alleviate exercise-induced fatigue. However, the exact mechanisms underlying its effects remain unclear. In the present study, we investigated the effects of Mongolian WA on iNOS/NO signaling pathway and proinflammatory cytokines in a chronic exhaustive swimming-induced fatigue rat model. Animals were randomly divided into Control group, Ctrl + WA group, Model group, and Model + WA group. The body weight, exhaustive swimming time test, and Morris water maze test were performed before and after the chronic exhaustive swimming. The serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and iNOS were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expressions of IL-1β, IL-6, TNF-α, IFN-γ, and iNOS in the hippocampus were measured by real-time polymerase chain reaction (RT-PCR). Moreover, the protein expression of iNOS in the hippocampus was measured by western blot, and the NO productions in the serum and hippocampus were detected by Griess reaction system. Chronic exhaustive exercise significantly reduced the body weight and exhaustive swimming time, and induced impairment in learning and memory, and which were reversed by WA treatment. Chronic exhaustive exercise also increased the expressions of iNOS and proinflammatory cytokines, while WA treatment significantly decreased the level of iNOS and proinflammatory cytokines. However, chronic exhaustive exercise did not affect the NO production. These findings demonstrated that WA could alleviate the chronic exhaustive swimming-induced fatigue and improve the learning and memory ability, and the actions might be related to the reduction of inflammatory response and iNOS expression.
诱导型一氧化氮合酶/一氧化氮（iNOS/NO）信号通路和炎性细胞因子在运动性疲劳的发病机制中起重要作用。研究发现，蒙医温针（WA）可缓解运动性疲劳。然而，其作用的确切机制尚不清楚。在本研究中，我们在慢性力竭游泳诱导的疲劳大鼠模型中研究了蒙医温针对iNOS/NO信号通路和促炎细胞因子的影响。将动物随机分为对照组、Ctrl + WA组、模型组和模型 + WA组。在慢性力竭游泳前后进行体重、力竭游泳时间测试和莫里斯水迷宫测试。采用酶联免疫吸附测定（ELISA）检测血清白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和iNOS水平。采用实时聚合酶链反应（RT-PCR）检测海马中IL-1β、IL-6、TNF-α、IFN-γ和iNOS的mRNA表达。此外，采用蛋白质印迹法检测海马中iNOS的蛋白表达，并用格里斯反应系统检测血清和海马中的NO生成。慢性力竭运动显著降低体重和力竭游泳时间，并导致学习和记忆障碍，而温针治疗可逆转这些变化。慢性力竭运动还增加了iNOS和促炎细胞因子的表达，而温针治疗显著降低了iNOS和促炎细胞因子的水平。然而，慢性力竭运动不影响NO的生成。这些结果表明，温针可缓解慢性力竭游泳诱导的疲劳并改善学习和记忆能力，其作用可能与减轻炎症反应和iNOS表达有关。
